Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
30958 | 5' | -48.9 | NC_006556.1 | + | 13464 | 0.66 | 0.743064 |
Target: 5'- ---aUGCCUcaAGcgGCAACGCCAcgauaacccuauAUGCu -3' miRNA: 3'- uuucACGGA--UCuaUGUUGCGGU------------UGCG- -5' |
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30958 | 5' | -48.9 | NC_006556.1 | + | 2417 | 0.67 | 0.706782 |
Target: 5'- ----aGCCUAGccGCcGCGUCAACGUc -3' miRNA: 3'- uuucaCGGAUCuaUGuUGCGGUUGCG- -5' |
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30958 | 5' | -48.9 | NC_006556.1 | + | 11288 | 0.67 | 0.694476 |
Target: 5'- ----cGCCUAGGUACAuaGUCAuaGCGCc -3' miRNA: 3'- uuucaCGGAUCUAUGUugCGGU--UGCG- -5' |
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30958 | 5' | -48.9 | NC_006556.1 | + | 11232 | 0.68 | 0.669646 |
Target: 5'- ---aUGCCUAGG----ACGCCAaaACGCg -3' miRNA: 3'- uuucACGGAUCUauguUGCGGU--UGCG- -5' |
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30958 | 5' | -48.9 | NC_006556.1 | + | 13604 | 0.7 | 0.545101 |
Target: 5'- ---uUGCCgucGUACAACGCCuauACGCc -3' miRNA: 3'- uuucACGGaucUAUGUUGCGGu--UGCG- -5' |
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30958 | 5' | -48.9 | NC_006556.1 | + | 14621 | 0.7 | 0.520865 |
Target: 5'- gAGAGUGCCggugUAGAUACGgguGgGCCAGC-Cg -3' miRNA: 3'- -UUUCACGG----AUCUAUGU---UgCGGUUGcG- -5' |
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30958 | 5' | -48.9 | NC_006556.1 | + | 7109 | 0.72 | 0.39693 |
Target: 5'- ---aUGCCgu-AUACAACGCCAugGUg -3' miRNA: 3'- uuucACGGaucUAUGUUGCGGUugCG- -5' |
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30958 | 5' | -48.9 | NC_006556.1 | + | 10707 | 1.1 | 0.001022 |
Target: 5'- cAAAGUGCCUAGAUACAACGCCAACGCc -3' miRNA: 3'- -UUUCACGGAUCUAUGUUGCGGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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