Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 30997 | 5' | -58.6 | NC_006560.1 | + | 77042 | 0.72 | 0.478046 |
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Target: 5'- uUCCgGGGCGCCCG-GGCC-CUCGUCc- -3' miRNA: 3'- -AGG-UCUGUGGGCaCCGGcGAGUAGca -5' |
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| 30997 | 5' | -58.6 | NC_006560.1 | + | 63910 | 0.72 | 0.459672 |
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Target: 5'- cUCCAGguacacggucACGCCCGUgGGCCGCaggcucuggUCGUUGUa -3' miRNA: 3'- -AGGUC----------UGUGGGCA-CCGGCG---------AGUAGCA- -5' |
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| 30997 | 5' | -58.6 | NC_006560.1 | + | 107012 | 0.72 | 0.450626 |
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Target: 5'- cCCGGGCcugcgaggGCCCGUcgcacaacgcgGaGCCGCUCGUCGUc -3' miRNA: 3'- aGGUCUG--------UGGGCA-----------C-CGGCGAGUAGCA- -5' |
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| 30997 | 5' | -58.6 | NC_006560.1 | + | 90857 | 0.73 | 0.432834 |
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Target: 5'- cCCGGGCcacgagggccgGCCCGcGGCCccGCUCGUCGg -3' miRNA: 3'- aGGUCUG-----------UGGGCaCCGG--CGAGUAGCa -5' |
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| 30997 | 5' | -58.6 | NC_006560.1 | + | 24315 | 0.74 | 0.365993 |
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Target: 5'- cUCCGGccGCGCCCucgGGCCGCUCcgCGc -3' miRNA: 3'- -AGGUC--UGUGGGca-CCGGCGAGuaGCa -5' |
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| 30997 | 5' | -58.6 | NC_006560.1 | + | 19578 | 0.74 | 0.358153 |
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Target: 5'- aCCGGGCgGCgCCGcGGcCCGCUCGUCGUc -3' miRNA: 3'- aGGUCUG-UG-GGCaCC-GGCGAGUAGCA- -5' |
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| 30997 | 5' | -58.6 | NC_006560.1 | + | 76111 | 0.74 | 0.358153 |
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Target: 5'- --aGGACGCCC-UGGCCGCgugCGUCGg -3' miRNA: 3'- aggUCUGUGGGcACCGGCGa--GUAGCa -5' |
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| 30997 | 5' | -58.6 | NC_006560.1 | + | 139913 | 1.07 | 0.002326 |
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Target: 5'- uUCCAGACACCCGUGGCCGCUCAUCGUc -3' miRNA: 3'- -AGGUCUGUGGGCACCGGCGAGUAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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