Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31004 | 5' | -60.5 | NC_006560.1 | + | 46735 | 0.71 | 0.40155 |
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Target: 5'- cGCCG-GGCCCGGUcgccuucgccguccUCcGCUCCCCGu -3' miRNA: 3'- cUGGCaCCGGGUCG--------------AGaUGAGGGGCu -5' |
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| 31004 | 5' | -60.5 | NC_006560.1 | + | 31619 | 0.72 | 0.379478 |
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Target: 5'- aGCCGcagugGGCCCGccGCUCccgcCUCCCCGAg -3' miRNA: 3'- cUGGCa----CCGGGU--CGAGau--GAGGGGCU- -5' |
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| 31004 | 5' | -60.5 | NC_006560.1 | + | 125087 | 0.72 | 0.355925 |
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Target: 5'- cGAUCGcccGGCCCGGCgugcgCUACUucgCCCCGGg -3' miRNA: 3'- -CUGGCa--CCGGGUCGa----GAUGA---GGGGCU- -5' |
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| 31004 | 5' | -60.5 | NC_006560.1 | + | 116889 | 0.73 | 0.311976 |
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Target: 5'- gGGCCGgucgucGGCCgccucgCGGCUCUGCcCCCCGAu -3' miRNA: 3'- -CUGGCa-----CCGG------GUCGAGAUGaGGGGCU- -5' |
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| 31004 | 5' | -60.5 | NC_006560.1 | + | 119575 | 0.73 | 0.311976 |
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Target: 5'- cGCCGgacagcgcccGGCCCcGCcCUGCUCCCCGGc -3' miRNA: 3'- cUGGCa---------CCGGGuCGaGAUGAGGGGCU- -5' |
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| 31004 | 5' | -60.5 | NC_006560.1 | + | 106248 | 0.74 | 0.291595 |
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Target: 5'- aGACCGcccgGGCCCGcggcauGCUCcgGCUCUCCGAc -3' miRNA: 3'- -CUGGCa---CCGGGU------CGAGa-UGAGGGGCU- -5' |
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| 31004 | 5' | -60.5 | NC_006560.1 | + | 141564 | 0.76 | 0.225773 |
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Target: 5'- cGCCGgcgGGCCCGGCUCga--CCCCGGc -3' miRNA: 3'- cUGGCa--CCGGGUCGAGaugaGGGGCU- -5' |
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| 31004 | 5' | -60.5 | NC_006560.1 | + | 70107 | 0.76 | 0.215252 |
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Target: 5'- -cCCGUGGCCCAGCU--GCcCCCCGu -3' miRNA: 3'- cuGGCACCGGGUCGAgaUGaGGGGCu -5' |
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| 31004 | 5' | -60.5 | NC_006560.1 | + | 128015 | 1.08 | 0.001263 |
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Target: 5'- cGACCGUGGCCCAGCUCUACUCCCCGAc -3' miRNA: 3'- -CUGGCACCGGGUCGAGAUGAGGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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