Results 1 - 20 of 88 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31006 | 3' | -58.3 | NC_006560.1 | + | 146513 | 0.66 | 0.825182 |
Target: 5'- gAGCUGcGCCGggcGGGCCGgcgcgUCGGGGg -3' miRNA: 3'- -UCGAC-CGGCaguUCUGGUa----GGCCCUg -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 70725 | 0.66 | 0.825182 |
Target: 5'- cGCgUGGCCG---AGuuCGUCCGGGAg -3' miRNA: 3'- uCG-ACCGGCaguUCugGUAGGCCCUg -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 66520 | 0.66 | 0.825182 |
Target: 5'- uAGUUGGCgGUgAcGugCGUCC-GGACg -3' miRNA: 3'- -UCGACCGgCAgUuCugGUAGGcCCUG- -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 69582 | 0.66 | 0.825182 |
Target: 5'- cGGCgcgGGCCGgcccAGcCCGcUCCGGGAg -3' miRNA: 3'- -UCGa--CCGGCagu-UCuGGU-AGGCCCUg -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 12861 | 0.66 | 0.816697 |
Target: 5'- cGUUGGCCGUCu---CCGUCaccucgggCGGGAg -3' miRNA: 3'- uCGACCGGCAGuucuGGUAG--------GCCCUg -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 138811 | 0.66 | 0.816697 |
Target: 5'- uGCgGcGCC-UgGGGACCA-CCGGGGCg -3' miRNA: 3'- uCGaC-CGGcAgUUCUGGUaGGCCCUG- -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 86276 | 0.66 | 0.808048 |
Target: 5'- cGCaUGGCCGc---GGCC-UCCGGGAa -3' miRNA: 3'- uCG-ACCGGCaguuCUGGuAGGCCCUg -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 130487 | 0.66 | 0.808048 |
Target: 5'- gGGCUGGUgGcgcggaUCGAGGCCGcgcCCGGcGGCc -3' miRNA: 3'- -UCGACCGgC------AGUUCUGGUa--GGCC-CUG- -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 35277 | 0.66 | 0.808048 |
Target: 5'- cGCgccgGGCgG-CGGGGCCcgggGUCCGGGGg -3' miRNA: 3'- uCGa---CCGgCaGUUCUGG----UAGGCCCUg -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 56556 | 0.66 | 0.808048 |
Target: 5'- gAGCgGaGCCG-CGGGAgCgagggucgGUCCGGGGCg -3' miRNA: 3'- -UCGaC-CGGCaGUUCUgG--------UAGGCCCUG- -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 84959 | 0.66 | 0.808048 |
Target: 5'- uGCUGuaCGUCAu--CCuccCCGGGACg -3' miRNA: 3'- uCGACcgGCAGUucuGGua-GGCCCUG- -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 67984 | 0.66 | 0.799243 |
Target: 5'- cGCgggGGCCG-CGGcGCCGUCgGGcGACg -3' miRNA: 3'- uCGa--CCGGCaGUUcUGGUAGgCC-CUG- -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 33205 | 0.66 | 0.799243 |
Target: 5'- aGGC-GGCCG-CGGGGCaga-CGGGGCa -3' miRNA: 3'- -UCGaCCGGCaGUUCUGguagGCCCUG- -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 98243 | 0.66 | 0.799243 |
Target: 5'- gGGCggGGUCGUCuccGCCGUCUcGGGCg -3' miRNA: 3'- -UCGa-CCGGCAGuucUGGUAGGcCCUG- -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 32410 | 0.66 | 0.799243 |
Target: 5'- gGGUgggGGCCGagGcccgcccaggGGGCCGgggggCCGGGGCu -3' miRNA: 3'- -UCGa--CCGGCagU----------UCUGGUa----GGCCCUG- -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 73779 | 0.66 | 0.796572 |
Target: 5'- cGGCUGGaCCGggugCGcgccgagggccagaAGGCCGUCgagGGGGCg -3' miRNA: 3'- -UCGACC-GGCa---GU--------------UCUGGUAGg--CCCUG- -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 138229 | 0.66 | 0.790289 |
Target: 5'- cGCggGGCCGUCG---UCGUCCGGcaGACg -3' miRNA: 3'- uCGa-CCGGCAGUucuGGUAGGCC--CUG- -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 5353 | 0.66 | 0.790289 |
Target: 5'- cGGCgGGCCGUCGu--CCGggUCCGGcGAg -3' miRNA: 3'- -UCGaCCGGCAGUucuGGU--AGGCC-CUg -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 104441 | 0.66 | 0.790289 |
Target: 5'- cGGCUGgcgcccGCCGUCGAGuCCcgCCGGc-- -3' miRNA: 3'- -UCGAC------CGGCAGUUCuGGuaGGCCcug -5' |
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31006 | 3' | -58.3 | NC_006560.1 | + | 28397 | 0.66 | 0.790289 |
Target: 5'- cGUUGGuCCG-CGgguuGGGCCGgggcgggCCGGGGCg -3' miRNA: 3'- uCGACC-GGCaGU----UCUGGUa------GGCCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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