miRNA: 3'- aCGCCUCGAGCGa--CGcGUGCauCaaC- -5'
miRNA: 3'- aCGCCUCGAGC-----GACG-CGUGCAUCaac -5'
miRNA: 3'- aCGCCuCGAGCGACGCGuGCauCaaC- -5'
miRNA: 3'- aCGCCUCGA--------------GCGAC-GCGUGCAUCAac -5'
miRNA: 3'- -ACGCCUCGAGCGACGCGUGCAUCAAC- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31021 | 5' | -56.4 | NC_006560.1 | + | 22331 | 0.74 | 0.424989 |
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Target: 5'- cGCGGAGCUCGCggaGCuCGCGgcGggGg -3' miRNA: 3'- aCGCCUCGAGCGa--CGcGUGCauCaaC- -5' |
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| 31021 | 5' | -56.4 | NC_006560.1 | + | 91322 | 0.75 | 0.37361 |
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Target: 5'- gGCGGAGCUCGagaacCUGCaGUACGUGGa-- -3' miRNA: 3'- aCGCCUCGAGC-----GACG-CGUGCAUCaac -5' |
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| 31021 | 5' | -56.4 | NC_006560.1 | + | 4714 | 0.76 | 0.357446 |
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Target: 5'- gGCGGcGCUCGCUGCGCcCGgcGgcGa -3' miRNA: 3'- aCGCCuCGAGCGACGCGuGCauCaaC- -5' |
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| 31021 | 5' | -56.4 | NC_006560.1 | + | 137650 | 0.78 | 0.275539 |
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Target: 5'- gGCGGAGCUgcuggaggagucgcCGCUGcCGUACGUGGUg- -3' miRNA: 3'- aCGCCUCGA--------------GCGAC-GCGUGCAUCAac -5' |
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| 31021 | 5' | -56.4 | NC_006560.1 | + | 109086 | 1.09 | 0.00235 |
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Target: 5'- cUGCGGAGCUCGCUGCGCACGUAGUUGg -3' miRNA: 3'- -ACGCCUCGAGCGACGCGUGCAUCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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