Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31302 | 5' | -58.1 | NC_006623.1 | + | 1768 | 1.11 | 0.00163 |
Target: 5'- cAGUAACUCGGUCUCCGGCGGGAGCGGg -3' miRNA: 3'- -UCAUUGAGCCAGAGGCCGCCCUCGCC- -5' |
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31302 | 5' | -58.1 | NC_006623.1 | + | 123114 | 0.68 | 0.743247 |
Target: 5'- cGUGucGCUC-GUUUCUGGgGGGGGgGGg -3' miRNA: 3'- uCAU--UGAGcCAGAGGCCgCCCUCgCC- -5' |
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31302 | 5' | -58.1 | NC_006623.1 | + | 124813 | 0.67 | 0.780285 |
Target: 5'- cGUGAUUCGGU--UCGGUgagGGGGGCGa -3' miRNA: 3'- uCAUUGAGCCAgaGGCCG---CCCUCGCc -5' |
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31302 | 5' | -58.1 | NC_006623.1 | + | 138498 | 0.68 | 0.743247 |
Target: 5'- cGUGucGCUC-GUUUCUGGgGGGGGgGGg -3' miRNA: 3'- uCAU--UGAGcCAGAGGCCgCCCUCgCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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