Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
31307 | 5' | -54.5 | NC_006623.1 | + | 2646 | 1.09 | 0.003833 |
Target: 5'- uUCACUGAGCCGACCAGAUCCACUGAGu -3' miRNA: 3'- -AGUGACUCGGCUGGUCUAGGUGACUC- -5' |
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31307 | 5' | -54.5 | NC_006623.1 | + | 2505 | 0.69 | 0.808576 |
Target: 5'- gCACUGAuCUGuCCAGGUgCACUGAa -3' miRNA: 3'- aGUGACUcGGCuGGUCUAgGUGACUc -5' |
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31307 | 5' | -54.5 | NC_006623.1 | + | 45702 | 0.66 | 0.930789 |
Target: 5'- gUACUgGAGCCGuACCAGAaugCCGgUGGu -3' miRNA: 3'- aGUGA-CUCGGC-UGGUCUa--GGUgACUc -5' |
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31307 | 5' | -54.5 | NC_006623.1 | + | 130517 | 0.66 | 0.94533 |
Target: 5'- -gACUGAGCCG-CCGGGg--ACUGAa -3' miRNA: 3'- agUGACUCGGCuGGUCUaggUGACUc -5' |
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31307 | 5' | -54.5 | NC_006623.1 | + | 45909 | 0.66 | 0.949692 |
Target: 5'- -uGCUGcuccuacaauGCCGGCCAauUCUGCUGAGa -3' miRNA: 3'- agUGACu---------CGGCUGGUcuAGGUGACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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