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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31308 | 5' | -54.8 | NC_006623.1 | + | 138894 | 0.69 | 0.774235 |
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Target: 5'- --cUUGCG-CgCCGG-CGCGGCUGCCg -3' miRNA: 3'- uuuGACGUaGgGGUCaGUGUCGACGG- -5' |
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| 31308 | 5' | -54.8 | NC_006623.1 | + | 125351 | 0.67 | 0.869893 |
|
Target: 5'- cAGCUGUAUUCUCuGUCACAGgUuCCg -3' miRNA: 3'- uUUGACGUAGGGGuCAGUGUCgAcGG- -5' |
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| 31308 | 5' | -54.8 | NC_006623.1 | + | 122718 | 0.69 | 0.774235 |
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Target: 5'- --cUUGCG-CgCCGG-CGCGGCUGCCg -3' miRNA: 3'- uuuGACGUaGgGGUCaGUGUCGACGG- -5' |
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| 31308 | 5' | -54.8 | NC_006623.1 | + | 77773 | 0.68 | 0.837755 |
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Target: 5'- gAGGCUGaCAU-UCUGGUCAC-GCUGCCa -3' miRNA: 3'- -UUUGAC-GUAgGGGUCAGUGuCGACGG- -5' |
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| 31308 | 5' | -54.8 | NC_006623.1 | + | 68664 | 0.67 | 0.85425 |
|
Target: 5'- uGAACUcuuCAUUCCCGGcCG-AGCUGCCa -3' miRNA: 3'- -UUUGAc--GUAGGGGUCaGUgUCGACGG- -5' |
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| 31308 | 5' | -54.8 | NC_006623.1 | + | 65650 | 0.66 | 0.923046 |
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Target: 5'- uGGACUGCAacaugaCCUAGaaACAGCUGUa -3' miRNA: 3'- -UUUGACGUag----GGGUCagUGUCGACGg -5' |
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| 31308 | 5' | -54.8 | NC_006623.1 | + | 34588 | 0.67 | 0.884632 |
|
Target: 5'- aAAACUGCAcaCCaCCGGaaguuuUCACGGC-GCCa -3' miRNA: 3'- -UUUGACGUa-GG-GGUC------AGUGUCGaCGG- -5' |
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| 31308 | 5' | -54.8 | NC_006623.1 | + | 31012 | 0.68 | 0.823986 |
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Target: 5'- gAGACUGCuUCUuugacggagccauagCCAGUUAUucguGCUGCCg -3' miRNA: 3'- -UUUGACGuAGG---------------GGUCAGUGu---CGACGG- -5' |
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| 31308 | 5' | -54.8 | NC_006623.1 | + | 22864 | 0.66 | 0.910619 |
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Target: 5'- -cGCgUGUAUCCauaaauuCCAGUCAgAGCUugaGCCg -3' miRNA: 3'- uuUG-ACGUAGG-------GGUCAGUgUCGA---CGG- -5' |
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| 31308 | 5' | -54.8 | NC_006623.1 | + | 2884 | 1.1 | 0.002297 |
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Target: 5'- aAAACUGCAUCCCCAGUCACAGCUGCCa -3' miRNA: 3'- -UUUGACGUAGGGGUCAGUGUCGACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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