Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31311 | 3' | -54.3 | NC_006623.1 | + | 121839 | 0.67 | 0.895062 |
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Target: 5'- -aGCggacGCGCGCCccuUUGCGacCGCCGGa -3' miRNA: 3'- aaCGa---CGUGUGGuu-AACGCa-GCGGCU- -5' |
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| 31311 | 3' | -54.3 | NC_006623.1 | + | 139772 | 0.67 | 0.895062 |
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Target: 5'- -aGCggacGCGCGCCccuUUGCGacCGCCGGa -3' miRNA: 3'- aaCGa---CGUGUGGuu-AACGCa-GCGGCU- -5' |
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| 31311 | 3' | -54.3 | NC_006623.1 | + | 7950 | 0.68 | 0.85815 |
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Target: 5'- -cGCUcacGCAgGCCGGU--CGUCGCCGGu -3' miRNA: 3'- aaCGA---CGUgUGGUUAacGCAGCGGCU- -5' |
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| 31311 | 3' | -54.3 | NC_006623.1 | + | 131224 | 0.68 | 0.841821 |
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Target: 5'- -cGCUcGCGCuCCGAgcGCGgCGCCGGa -3' miRNA: 3'- aaCGA-CGUGuGGUUaaCGCaGCGGCU- -5' |
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| 31311 | 3' | -54.3 | NC_006623.1 | + | 111855 | 0.69 | 0.806796 |
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Target: 5'- aUUGaUGUACugC-AUUGCGUCGCgGAa -3' miRNA: 3'- -AACgACGUGugGuUAACGCAGCGgCU- -5' |
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| 31311 | 3' | -54.3 | NC_006623.1 | + | 31122 | 0.74 | 0.55164 |
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Target: 5'- -aGCcGUACACgucuaAAUUGCGUUGCCGAg -3' miRNA: 3'- aaCGaCGUGUGg----UUAACGCAGCGGCU- -5' |
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| 31311 | 3' | -54.3 | NC_006623.1 | + | 8163 | 1.07 | 0.004436 |
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Target: 5'- uUUGCUGCACACCAAUUGCGUCGCCGAg -3' miRNA: 3'- -AACGACGUGUGGUUAACGCAGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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