Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31313 | 3' | -51.9 | NC_006623.1 | + | 139487 | 0.66 | 0.984488 |
Target: 5'- gGGAUguGUcuagaggagAGCGGUCCGGGCGGu--- -3' miRNA: 3'- -UCUAguCG---------UUGCCGGGCCUGUUuucu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 122125 | 0.66 | 0.984488 |
Target: 5'- gGGAUguGUcuagaggagAGCGGUCCGGGCGGu--- -3' miRNA: 3'- -UCUAguCG---------UUGCCGGGCCUGUUuucu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 123621 | 0.66 | 0.980416 |
Target: 5'- ---cCAcGCGGCGGCCCGGGgAAu--- -3' miRNA: 3'- ucuaGU-CGUUGCCGGGCCUgUUuucu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 136582 | 0.66 | 0.980416 |
Target: 5'- cAGAggGGCcgaagacGCGGCCgCGGGCAAGAa- -3' miRNA: 3'- -UCUagUCGu------UGCCGG-GCCUGUUUUcu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 137991 | 0.66 | 0.980416 |
Target: 5'- ---cCAcGCGGCGGCCCGGGgAAu--- -3' miRNA: 3'- ucuaGU-CGUUGCCGGGCCUgUUuucu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 101636 | 0.66 | 0.980416 |
Target: 5'- cAGG--GGCAGCGGCCCauugauaauGCAGAAGAu -3' miRNA: 3'- -UCUagUCGUUGCCGGGcc-------UGUUUUCU- -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 124827 | 0.66 | 0.978103 |
Target: 5'- aGGAUguGCAGacccgugauuCGGUUCGGugAGGGGGg -3' miRNA: 3'- -UCUAguCGUU----------GCCGGGCCugUUUUCU- -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 27054 | 0.67 | 0.972883 |
Target: 5'- gAGAaauugCAGCGGCGGCacuaCCGGcCAGAaaAGAu -3' miRNA: 3'- -UCUa----GUCGUUGCCG----GGCCuGUUU--UCU- -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 27830 | 0.67 | 0.966819 |
Target: 5'- cAGAUUuuaCAACGGCCgGGGCGcAAGu -3' miRNA: 3'- -UCUAGuc-GUUGCCGGgCCUGUuUUCu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 137448 | 0.68 | 0.955621 |
Target: 5'- ---cCGGCAGCGGCCgccgacgCGGcgaGCGGAAGAc -3' miRNA: 3'- ucuaGUCGUUGCCGG-------GCC---UGUUUUCU- -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 146841 | 0.68 | 0.938221 |
Target: 5'- ---gCAGCGACGGCCCuaaaGGaaGCGGAAGu -3' miRNA: 3'- ucuaGUCGUUGCCGGG----CC--UGUUUUCu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 114770 | 0.69 | 0.933142 |
Target: 5'- ---cCAGCGACGGCCCuaaaGGaaGCGGAAGu -3' miRNA: 3'- ucuaGUCGUUGCCGGG----CC--UGUUUUCu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 1242 | 0.69 | 0.927807 |
Target: 5'- cAGAUCAGac-CGGCCUGGcACGGAcccccAGAg -3' miRNA: 3'- -UCUAGUCguuGCCGGGCC-UGUUU-----UCU- -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 111829 | 0.69 | 0.922217 |
Target: 5'- ----uGGCAGCGGCCCGG-CGGGGc- -3' miRNA: 3'- ucuagUCGUUGCCGGGCCuGUUUUcu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 55065 | 0.69 | 0.922217 |
Target: 5'- uAGAUCAGCA--GGCCCGcAUAGcGGAu -3' miRNA: 3'- -UCUAGUCGUugCCGGGCcUGUUuUCU- -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 145520 | 0.72 | 0.827361 |
Target: 5'- cGGGUUgAGCAGCGGUCCGG-CGGGAa- -3' miRNA: 3'- -UCUAG-UCGUUGCCGGGCCuGUUUUcu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 116092 | 0.72 | 0.827361 |
Target: 5'- cGGGUUgAGCAGCGGUCCGG-CGGGAa- -3' miRNA: 3'- -UCUAG-UCGUUGCCGGGCCuGUUUUcu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 20195 | 0.73 | 0.771886 |
Target: 5'- uGA-CGGCAAUGGCCCaGGCAGAAu- -3' miRNA: 3'- uCUaGUCGUUGCCGGGcCUGUUUUcu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 38075 | 0.74 | 0.721903 |
Target: 5'- gAGAgUCAGCAACcGUCCGGGCAAAc-- -3' miRNA: 3'- -UCU-AGUCGUUGcCGGGCCUGUUUucu -5' |
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31313 | 3' | -51.9 | NC_006623.1 | + | 45059 | 0.75 | 0.62756 |
Target: 5'- aAGAUCAGCcuuuAACGGgaugCUGGACAAAGGAu -3' miRNA: 3'- -UCUAGUCG----UUGCCg---GGCCUGUUUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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