Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31317 | 5' | -53.6 | NC_006623.1 | + | 137280 | 0.74 | 0.637431 |
Target: 5'- ------cGCGuCGGCGGcCGCUGCCGGa -3' miRNA: 3'- ucuuuaaCGUcGCCGCC-GUGAUGGCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 137735 | 0.7 | 0.844496 |
Target: 5'- ------cGCAGCGGCGGUaggacuguggaccgcGCUGUCGGa -3' miRNA: 3'- ucuuuaaCGUCGCCGCCG---------------UGAUGGCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 139573 | 0.69 | 0.880008 |
Target: 5'- aAGggGggGgGGCGGCGGgAUguCCGGc -3' miRNA: 3'- -UCuuUaaCgUCGCCGCCgUGauGGCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 139943 | 0.72 | 0.740006 |
Target: 5'- gGGGGAUaGCAaaugGCGGCGGC---GCCGGa -3' miRNA: 3'- -UCUUUAaCGU----CGCCGCCGugaUGGCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 140081 | 0.67 | 0.930063 |
Target: 5'- cGuuAUUGCGGUGcGCGGCAC--CCGc -3' miRNA: 3'- uCuuUAACGUCGC-CGCCGUGauGGCc -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 144329 | 0.67 | 0.944774 |
Target: 5'- uGGAAGccGCAGCGaaagagccaccuGCGGCAaccCCGGa -3' miRNA: 3'- -UCUUUaaCGUCGC------------CGCCGUgauGGCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 144830 | 0.67 | 0.940116 |
Target: 5'- aAGAcgagGCGGcCGGCGGaCGacUUGCCGGu -3' miRNA: 3'- -UCUuuaaCGUC-GCCGCC-GU--GAUGGCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 145455 | 0.66 | 0.960997 |
Target: 5'- cGGGGAUUGUGGUucuuGCGGUuCUugCGGu -3' miRNA: 3'- -UCUUUAACGUCGc---CGCCGuGAugGCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 148036 | 0.67 | 0.944774 |
Target: 5'- uGGGGcaUGCGcGCGGCcggccuccagGGCAUUGCCGu -3' miRNA: 3'- -UCUUuaACGU-CGCCG----------CCGUGAUGGCc -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 148606 | 0.72 | 0.699609 |
Target: 5'- gAGAAGUUucGCGGCGGCGGUugcGCgCGGu -3' miRNA: 3'- -UCUUUAA--CGUCGCCGCCGugaUG-GCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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