Results 21 - 30 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31317 | 5' | -53.6 | NC_006623.1 | + | 144329 | 0.67 | 0.944774 |
Target: 5'- uGGAAGccGCAGCGaaagagccaccuGCGGCAaccCCGGa -3' miRNA: 3'- -UCUUUaaCGUCGC------------CGCCGUgauGGCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 148036 | 0.67 | 0.944774 |
Target: 5'- uGGGGcaUGCGcGCGGCcggccuccagGGCAUUGCCGu -3' miRNA: 3'- -UCUUuaACGU-CGCCG----------CCGUGAUGGCc -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 111834 | 0.66 | 0.949187 |
Target: 5'- uGAAAUgGCAGCGGCccGGCGggGCaUGGc -3' miRNA: 3'- uCUUUAaCGUCGCCG--CCGUgaUG-GCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 66553 | 0.66 | 0.949187 |
Target: 5'- uGAGuuUUGUAGCuGCGGCcggcgcggcucACUACCGa -3' miRNA: 3'- uCUUu-AACGUCGcCGCCG-----------UGAUGGCc -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 132439 | 0.66 | 0.957295 |
Target: 5'- -uGGAUUGCGGUGGaaaaCGGUGCUGCUcaGGc -3' miRNA: 3'- ucUUUAACGUCGCC----GCCGUGAUGG--CC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 116157 | 0.66 | 0.960997 |
Target: 5'- cGGGGAUUGUGGUucuuGCGGUuCUugCGGu -3' miRNA: 3'- -UCUUUAACGUCGc---CGCCGuGAugGCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 105793 | 0.66 | 0.960997 |
Target: 5'- uAGAGuUUGCGGCaa-GGCA-UGCCGGa -3' miRNA: 3'- -UCUUuAACGUCGccgCCGUgAUGGCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 145455 | 0.66 | 0.960997 |
Target: 5'- cGGGGAUUGUGGUucuuGCGGUuCUugCGGu -3' miRNA: 3'- -UCUUUAACGUCGc---CGCCGuGAugGCC- -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 25712 | 0.66 | 0.967723 |
Target: 5'- uAGAAGcgGUGGCGGgGGCGCgcaaauCCa- -3' miRNA: 3'- -UCUUUaaCGUCGCCgCCGUGau----GGcc -5' |
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31317 | 5' | -53.6 | NC_006623.1 | + | 80857 | 0.66 | 0.967723 |
Target: 5'- uGAGAUUGCuccuGCGGuCGGaGCc-CCGGa -3' miRNA: 3'- uCUUUAACGu---CGCC-GCCgUGauGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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