miRNA display CGI


Results 1 - 12 of 12 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
3132 3' -51.9 NC_001493.1 + 29719 0.66 0.98689
Target:  5'- cUCUCGAGCUGgcgguucgccuUCGagagguccauCACGGugGUCCCGa -3'
miRNA:   3'- -AGAGCUUGAC-----------AGCau--------GUGCC--UAGGGU- -5'
3132 3' -51.9 NC_001493.1 + 60458 0.66 0.98689
Target:  5'- --gUGGACggUGUCGUGCACGGGgggugucaUCCUc -3'
miRNA:   3'- agaGCUUG--ACAGCAUGUGCCU--------AGGGu -5'
3132 3' -51.9 NC_001493.1 + 50253 0.66 0.985185
Target:  5'- aUCcCGAAUaagUGgauGUACugGGAUCCCu -3'
miRNA:   3'- -AGaGCUUG---ACag-CAUGugCCUAGGGu -5'
3132 3' -51.9 NC_001493.1 + 67018 0.66 0.978349
Target:  5'- aCUCGAugUGuuUCGUGCcgaucagccccgggAUGGAUgCCAg -3'
miRNA:   3'- aGAGCUugAC--AGCAUG--------------UGCCUAgGGU- -5'
3132 3' -51.9 NC_001493.1 + 46605 0.67 0.964935
Target:  5'- gUUCGAGaCUGUCGUAaauguuCGGGcaugUCCCGa -3'
miRNA:   3'- aGAGCUU-GACAGCAUgu----GCCU----AGGGU- -5'
3132 3' -51.9 NC_001493.1 + 28604 0.69 0.924983
Target:  5'- aUCUCGGucgcgugcCUGUCGgugagcGCACGGAUCUg- -3'
miRNA:   3'- -AGAGCUu-------GACAGCa-----UGUGCCUAGGgu -5'
3132 3' -51.9 NC_001493.1 + 51600 0.69 0.913362
Target:  5'- gUCUUGAACgggGUCaugaACAUGGGUCUCAg -3'
miRNA:   3'- -AGAGCUUGa--CAGca--UGUGCCUAGGGU- -5'
3132 3' -51.9 NC_001493.1 + 18785 0.72 0.832316
Target:  5'- gCUCGAcgcgGCcGUCGUACuCGGggCCCGu -3'
miRNA:   3'- aGAGCU----UGaCAGCAUGuGCCuaGGGU- -5'
3132 3' -51.9 NC_001493.1 + 94947 0.73 0.748511
Target:  5'- cCUCGGGCUGUUaaaugACACGGAgcuccgCCCGg -3'
miRNA:   3'- aGAGCUUGACAGca---UGUGCCUa-----GGGU- -5'
3132 3' -51.9 NC_001493.1 + 31479 0.76 0.592923
Target:  5'- gUUCGuAugUGUCGUGCgggAUGGGUCCCAu -3'
miRNA:   3'- aGAGC-UugACAGCAUG---UGCCUAGGGU- -5'
3132 3' -51.9 NC_001493.1 + 8964 0.96 0.047419
Target:  5'- aUCUCGAACUGUCGUACACG-AUCCCAc -3'
miRNA:   3'- -AGAGCUUGACAGCAUGUGCcUAGGGU- -5'
3132 3' -51.9 NC_001493.1 + 124518 1.1 0.006353
Target:  5'- aUCUCGAACUGUCGUACACGGAUCCCAc -3'
miRNA:   3'- -AGAGCUUGACAGCAUGUGCCUAGGGU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.