miRNA: 3'- -GGCU---CGGUUaUuuCUUaaaGGGACCG- -5'
miRNA: 3'- -GGCUcGgUUAUUUCuuaaAGGGACCg -5'
miRNA: 3'- ggCUCgg-UUAUUUCuuaaa-GGGACCG- -5'
miRNA: 3'- -GGCUCGguuaUUUCU-------UAaAG-GGACCG- -5'
miRNA: 3'- gGCUCGGUUAuUUCUUaaAGGGaccg -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31321 | 3' | -46.1 | NC_006623.1 | + | 127820 | 0.68 | 0.999762 |
|
Target: 5'- aCCGAaacGCUGAaAgcGAGgcgCCCUGGCa -3' miRNA: 3'- -GGCU---CGGUUaUuuCUUaaaGGGACCG- -5' |
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| 31321 | 3' | -46.1 | NC_006623.1 | + | 130771 | 0.68 | 0.999514 |
|
Target: 5'- cCCGGaCgGAUAGAGucg-UCCCUGGg -3' miRNA: 3'- -GGCUcGgUUAUUUCuuaaAGGGACCg -5' |
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| 31321 | 3' | -46.1 | NC_006623.1 | + | 138494 | 0.67 | 0.999937 |
|
Target: 5'- -aGAGaaaGAUAGAGcgcgcgCCCUGGCg -3' miRNA: 3'- ggCUCgg-UUAUUUCuuaaa-GGGACCG- -5' |
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| 31321 | 3' | -46.1 | NC_006623.1 | + | 139070 | 0.66 | 0.999952 |
|
Target: 5'- gCCGAGCg---AGAGAccucccgGUcUCuCCUGGCg -3' miRNA: 3'- -GGCUCGguuaUUUCU-------UAaAG-GGACCG- -5' |
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| 31321 | 3' | -46.1 | NC_006623.1 | + | 142917 | 0.68 | 0.999762 |
|
Target: 5'- gCGGGCCAGUuGAGAA--UCCCg--- -3' miRNA: 3'- gGCUCGGUUAuUUCUUaaAGGGaccg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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