Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31321 | 3' | -46.1 | NC_006623.1 | + | 122542 | 0.66 | 0.999952 |
Target: 5'- gCCGAGCg---AGAGAccucccgGUcUCuCCUGGCg -3' miRNA: 3'- -GGCUCGguuaUUUCU-------UAaAG-GGACCG- -5' |
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31321 | 3' | -46.1 | NC_006623.1 | + | 83475 | 0.66 | 0.999953 |
Target: 5'- -aGAGCUGAagauAAGAGUcggUgCCUGGCu -3' miRNA: 3'- ggCUCGGUUau--UUCUUAa--AgGGACCG- -5' |
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31321 | 3' | -46.1 | NC_006623.1 | + | 90368 | 0.66 | 0.999953 |
Target: 5'- -gGAGUCGGUGGAG----UUCUUGGCg -3' miRNA: 3'- ggCUCGGUUAUUUCuuaaAGGGACCG- -5' |
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31321 | 3' | -46.1 | NC_006623.1 | + | 68530 | 0.66 | 0.999975 |
Target: 5'- -aGAGCCuu--AAGAAa-UCCCaGGCa -3' miRNA: 3'- ggCUCGGuuauUUCUUaaAGGGaCCG- -5' |
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31321 | 3' | -46.1 | NC_006623.1 | + | 97030 | 0.66 | 0.999982 |
Target: 5'- --aAGCCA--AAAGAcaUUUCCUGGUa -3' miRNA: 3'- ggcUCGGUuaUUUCUuaAAGGGACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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