Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31335 | 3' | -61.7 | NC_006623.1 | + | 46257 | 0.67 | 0.583303 |
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Target: 5'- cGGCGcGcCGCCUguagcuugGCUGCUgaacuUGCGCUgGCa -3' miRNA: 3'- -UCGC-C-GUGGAa-------CGGCGG-----ACGCGAgCG- -5' |
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| 31335 | 3' | -61.7 | NC_006623.1 | + | 107520 | 0.66 | 0.603091 |
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Target: 5'- aGGgGGUGCCguauaccGCCcaGCUUGCGC-CGCa -3' miRNA: 3'- -UCgCCGUGGaa-----CGG--CGGACGCGaGCG- -5' |
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| 31335 | 3' | -61.7 | NC_006623.1 | + | 122890 | 0.66 | 0.626939 |
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Target: 5'- uGGCGGCAgCCgcGCCGgC-GCGCaaggcggccagguauUCGCg -3' miRNA: 3'- -UCGCCGU-GGaaCGGCgGaCGCG---------------AGCG- -5' |
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| 31335 | 3' | -61.7 | NC_006623.1 | + | 9832 | 0.66 | 0.652795 |
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Target: 5'- aAGCGGCugUUUGCUcaUCUGCacCUCGUg -3' miRNA: 3'- -UCGCCGugGAACGGc-GGACGc-GAGCG- -5' |
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| 31335 | 3' | -61.7 | NC_006623.1 | + | 127485 | 0.66 | 0.652795 |
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Target: 5'- cGCGGCguccaagacACCUcGCgcuucgaGCCUGCGCUgGg -3' miRNA: 3'- uCGCCG---------UGGAaCGg------CGGACGCGAgCg -5' |
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| 31335 | 3' | -61.7 | NC_006623.1 | + | 7857 | 0.66 | 0.652795 |
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Target: 5'- gGGCGGguUUggGCCaCCgugGCGgUCGCa -3' miRNA: 3'- -UCGCCguGGaaCGGcGGa--CGCgAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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