Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31335 | 3' | -61.7 | NC_006623.1 | + | 133803 | 0.71 | 0.329395 |
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Target: 5'- -cUGGCucuCCUUGCCGCga-CGCUCGCa -3' miRNA: 3'- ucGCCGu--GGAACGGCGgacGCGAGCG- -5' |
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| 31335 | 3' | -61.7 | NC_006623.1 | + | 144544 | 0.72 | 0.315137 |
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Target: 5'- gAGC-GCGCCaUGCCGUaCUGCGCggcuUCGCu -3' miRNA: 3'- -UCGcCGUGGaACGGCG-GACGCG----AGCG- -5' |
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| 31335 | 3' | -61.7 | NC_006623.1 | + | 117068 | 0.72 | 0.315137 |
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Target: 5'- gAGC-GCGCCaUGCCGUaCUGCGCggcuUCGCu -3' miRNA: 3'- -UCGcCGUGGaACGGCG-GACGCG----AGCG- -5' |
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| 31335 | 3' | -61.7 | NC_006623.1 | + | 491 | 0.76 | 0.17859 |
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Target: 5'- uGUGGCugCUgugcGCUGCCgcgcuuuuaaugGCGCUCGCg -3' miRNA: 3'- uCGCCGugGAa---CGGCGGa-----------CGCGAGCG- -5' |
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| 31335 | 3' | -61.7 | NC_006623.1 | + | 139378 | 1.1 | 0.000694 |
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Target: 5'- cAGCGGCACCUUGCCGCCUGCGCUCGCc -3' miRNA: 3'- -UCGCCGUGGAACGGCGGACGCGAGCG- -5' |
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| 31335 | 3' | -61.7 | NC_006623.1 | + | 122234 | 1.1 | 0.000694 |
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Target: 5'- cAGCGGCACCUUGCCGCCUGCGCUCGCc -3' miRNA: 3'- -UCGCCGUGGAACGGCGGACGCGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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