Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31345 | 5' | -61.7 | NC_006623.1 | + | 97186 | 0.74 | 0.271036 |
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Target: 5'- uUCUCGacuaaguuuCCGGACUGCUGCgUgGACCCGUu -3' miRNA: 3'- -AGGGC---------GGCCUGGCGACG-AgUUGGGCG- -5' |
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| 31345 | 5' | -61.7 | NC_006623.1 | + | 7755 | 0.74 | 0.269197 |
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Target: 5'- gUCCCGCCGG-UCGCUGUagauacauugUCAuagucagaguccccGCCCGUa -3' miRNA: 3'- -AGGGCGGCCuGGCGACG----------AGU--------------UGGGCG- -5' |
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| 31345 | 5' | -61.7 | NC_006623.1 | + | 111664 | 0.85 | 0.047553 |
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Target: 5'- cCCCGCCGGGCCGCUGC-CAuuucaacgaacggGCCCGa -3' miRNA: 3'- aGGGCGGCCUGGCGACGaGU-------------UGGGCg -5' |
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| 31345 | 5' | -61.7 | NC_006623.1 | + | 115919 | 1.11 | 0.000615 |
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Target: 5'- uUCCCGCCGGACCGCUGCUCAACCCGCg -3' miRNA: 3'- -AGGGCGGCCUGGCGACGAGUUGGGCG- -5' |
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| 31345 | 5' | -61.7 | NC_006623.1 | + | 145693 | 1.11 | 0.000615 |
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Target: 5'- uUCCCGCCGGACCGCUGCUCAACCCGCg -3' miRNA: 3'- -AGGGCGGCCUGGCGACGAGUUGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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