miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
3151 5' -55.7 NC_001499.1 + 3836 0.66 0.141872
Target:  5'- aGUC-UUCAGAgcaGGAGACCCaUCCaCAGGc -3'
miRNA:   3'- -UAGuGAGUCU---CCUCUGGG-AGG-GUUCc -5'
3151 5' -55.7 NC_001499.1 + 5090 0.67 0.116242
Target:  5'- aGUCACUCGGAGGAcuGGCgCg-CCGAGu -3'
miRNA:   3'- -UAGUGAGUCUCCU--CUGgGagGGUUCc -5'
3151 5' -55.7 NC_001499.1 + 150 0.7 0.067517
Target:  5'- --gGCUCguccgGGAucgGGAGACCCcugCCCAGGGa -3'
miRNA:   3'- uagUGAG-----UCU---CCUCUGGGa--GGGUUCC- -5'
3151 5' -55.7 NC_001499.1 + 2795 0.78 0.013983
Target:  5'- cUC-CUCAGAGGAGGCCUUgCUGAGGc -3'
miRNA:   3'- uAGuGAGUCUCCUCUGGGAgGGUUCC- -5'
3151 5' -55.7 NC_001499.1 + 5186 1.09 0.000029
Target:  5'- aAUCACUCAGAGGAGACCCUCCCAAGGa -3'
miRNA:   3'- -UAGUGAGUCUCCUCUGGGAGGGUUCC- -5'
3151 5' -55.7 NC_001499.1 + 122 1.09 0.000029
Target:  5'- aAUCACUCAGAGGAGACCCUCCCAAGGa -3'
miRNA:   3'- -UAGUGAGUCUCCUCUGGGAGGGUUCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.