Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3160 | 3' | -53 | NC_001500.1 | + | 3997 | 0.66 | 0.256292 |
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Target: 5'- cCGGAauacucCGGGCCCCCAu-----GGACUUa -3' miRNA: 3'- -GCCU------GCUCGGGGGUuuacuuUCUGGG- -5' |
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| 3160 | 3' | -53 | NC_001500.1 | + | 5107 | 0.66 | 0.256291 |
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Target: 5'- gGGauGCGGGgCCCUAuAUuGAGGACCUg -3' miRNA: 3'- gCC--UGCUCgGGGGUuUAcUUUCUGGG- -5' |
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| 3160 | 3' | -53 | NC_001500.1 | + | 842 | 0.66 | 0.248547 |
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Target: 5'- aGGAgGGGCCCcaaauaguCCGAAUcGGucAGGACUCg -3' miRNA: 3'- gCCUgCUCGGG--------GGUUUA-CU--UUCUGGG- -5' |
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| 3160 | 3' | -53 | NC_001500.1 | + | 2042 | 0.67 | 0.206079 |
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Target: 5'- uGGugGGGCUucugCCCGcggucuGGAGACCCg -3' miRNA: 3'- gCCugCUCGG----GGGUuuac--UUUCUGGG- -5' |
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| 3160 | 3' | -53 | NC_001500.1 | + | 792 | 0.67 | 0.192766 |
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Target: 5'- gGGccacccaGCGGGCCCCCGAugccucUGAGAcGucuCCCa -3' miRNA: 3'- gCC-------UGCUCGGGGGUUu-----ACUUU-Cu--GGG- -5' |
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| 3160 | 3' | -53 | NC_001500.1 | + | 1299 | 0.69 | 0.141591 |
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Target: 5'- aCGGAgGggguucauaggcaauAGCCuCCCAGGUGAcaauauauGGGACCUg -3' miRNA: 3'- -GCCUgC---------------UCGG-GGGUUUACU--------UUCUGGG- -5' |
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| 3160 | 3' | -53 | NC_001500.1 | + | 536 | 1.12 | 0.000048 |
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Target: 5'- cCGGACGAGCCCCCAAAUGAAAGACCCc -3' miRNA: 3'- -GCCUGCUCGGGGGUUUACUUUCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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