Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31608 | 5' | -60.1 | NC_006879.1 | + | 33728 | 0.66 | 0.333247 |
Target: 5'- aGCAGCAGGcaAGCaacCGGCUGuccccgggaaCCGCGGu -3' miRNA: 3'- cCGUCGUCU--UCGcc-GUCGAC----------GGCGCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 12941 | 0.66 | 0.325181 |
Target: 5'- uGCGGCGGAaccAGCGGUcgcGGCUGaCUGUc- -3' miRNA: 3'- cCGUCGUCU---UCGCCG---UCGAC-GGCGcc -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 17314 | 0.66 | 0.317261 |
Target: 5'- cGGCuGGCAGAGGCa-CGGCgacgGgUGCGGa -3' miRNA: 3'- -CCG-UCGUCUUCGccGUCGa---CgGCGCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 13107 | 0.66 | 0.309487 |
Target: 5'- cGCGGCAGAaugagaagAGCGGUgagAGUcacGCCgGCGGc -3' miRNA: 3'- cCGUCGUCU--------UCGCCG---UCGa--CGG-CGCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 22182 | 0.67 | 0.301858 |
Target: 5'- cGGUGcGCAGAGuacgucgcGCGGaCGGCU-CCGUGGa -3' miRNA: 3'- -CCGU-CGUCUU--------CGCC-GUCGAcGGCGCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 10123 | 0.67 | 0.301858 |
Target: 5'- cGCAuCGGGAGCGGCGGauccgGCCGgucucgaacCGGg -3' miRNA: 3'- cCGUcGUCUUCGCCGUCga---CGGC---------GCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 21143 | 0.67 | 0.299599 |
Target: 5'- cGGCAGUuuuGGAAGCagaaccuagaagacGuGCccCUGCCGCGGc -3' miRNA: 3'- -CCGUCG---UCUUCG--------------C-CGucGACGGCGCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 22216 | 0.67 | 0.279847 |
Target: 5'- uGGCGGCGGc-GCGGguGUcGCCuuGCGa -3' miRNA: 3'- -CCGUCGUCuuCGCCguCGaCGG--CGCc -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 8092 | 0.67 | 0.265894 |
Target: 5'- gGGCAGCGGAGGCaguGGCAcGUUuUCGUGa -3' miRNA: 3'- -CCGUCGUCUUCG---CCGU-CGAcGGCGCc -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 9756 | 0.68 | 0.259131 |
Target: 5'- gGGcCAGCcGAcuguGGCGGgGGC-GCCGgGGg -3' miRNA: 3'- -CC-GUCGuCU----UCGCCgUCGaCGGCgCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 14998 | 0.68 | 0.252509 |
Target: 5'- uGGCGGCGGuggGGCGacgucuccgauGCAGCcGCCucuuGCGGc -3' miRNA: 3'- -CCGUCGUCu--UCGC-----------CGUCGaCGG----CGCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 15001 | 0.68 | 0.252509 |
Target: 5'- gGGUAgGCAGAAG-GGCuauGC-GCCGCGc -3' miRNA: 3'- -CCGU-CGUCUUCgCCGu--CGaCGGCGCc -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 2707 | 0.69 | 0.221471 |
Target: 5'- aGCuGCgAGggGCacaGGCcGCUGCCGCa- -3' miRNA: 3'- cCGuCG-UCuuCG---CCGuCGACGGCGcc -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 4460 | 0.69 | 0.221471 |
Target: 5'- uGGUAGUucagAGAGGaa-CAGCUGCCGuCGGg -3' miRNA: 3'- -CCGUCG----UCUUCgccGUCGACGGC-GCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 16666 | 0.69 | 0.215667 |
Target: 5'- aGguGCccauGAGCGGCcgcgAGCcgUGCCGCGGa -3' miRNA: 3'- cCguCGuc--UUCGCCG----UCG--ACGGCGCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 11080 | 0.69 | 0.20445 |
Target: 5'- aGGCGGU--GAGCGcCAGCcGCCgGCGGg -3' miRNA: 3'- -CCGUCGucUUCGCcGUCGaCGG-CGCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 19101 | 0.69 | 0.199034 |
Target: 5'- cGGUAGCGGAGucCGGC-GUUGCgGUGGu -3' miRNA: 3'- -CCGUCGUCUUc-GCCGuCGACGgCGCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 21157 | 0.69 | 0.199034 |
Target: 5'- cGCAGUuagggccGGAGCucccGCGGCUGuuGCGGa -3' miRNA: 3'- cCGUCGu------CUUCGc---CGUCGACggCGCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 11309 | 0.69 | 0.199034 |
Target: 5'- gGGCAGguGuuguuuuAGauGCAGCgGCCgGCGGa -3' miRNA: 3'- -CCGUCguCu------UCgcCGUCGaCGG-CGCC- -5' |
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31608 | 5' | -60.1 | NC_006879.1 | + | 21846 | 0.69 | 0.193742 |
Target: 5'- uGGUuGguGAagguuAGCGGCAG--GCCGCGGu -3' miRNA: 3'- -CCGuCguCU-----UCGCCGUCgaCGGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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