Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31660 | 3' | -57.1 | NC_006883.1 | + | 23302 | 0.66 | 0.94842 |
Target: 5'- aGUUGGACCaccgauaccUG-GaGGACCU-UGaGGACCa -3' miRNA: 3'- -CAACCUGG---------ACaC-CCUGGAcAC-CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 26672 | 0.66 | 0.944235 |
Target: 5'- -cUGGACCUG-GaGGACC-GUcuGGACCn -3' miRNA: 3'- caACCUGGACaC-CCUGGaCAc-CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 26773 | 0.68 | 0.890408 |
Target: 5'- aGUUGGACCUGcaGGACCaGUugcaccagcaGGACCa -3' miRNA: 3'- -CAACCUGGACacCCUGGaCAc---------CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 223073 | 0.68 | 0.883843 |
Target: 5'- --aGGACCaG-GaGGACCUG-GaGGACCa -3' miRNA: 3'- caaCCUGGaCaC-CCUGGACaC-CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 28459 | 0.68 | 0.862936 |
Target: 5'- --aGGACCUG-GaGGuCCUG-GaGGACCa -3' miRNA: 3'- caaCCUGGACaC-CCuGGACaC-CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 27543 | 0.69 | 0.855579 |
Target: 5'- -cUGGACCUG-GaGGACCaucuccaccUGUaGGACCu -3' miRNA: 3'- caACCUGGACaC-CCUGG---------ACAcCCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 23693 | 0.69 | 0.848035 |
Target: 5'- -aUGGACCUG-GaGGACCaGcaGGACCa -3' miRNA: 3'- caACCUGGACaC-CCUGGaCacCCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 22174 | 0.69 | 0.833211 |
Target: 5'- --aGGACCUGgaggaccaccacuaGGACCUG-GaGGACCa -3' miRNA: 3'- caaCCUGGACac------------CCUGGACaC-CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 22221 | 0.69 | 0.824349 |
Target: 5'- cUUGGACCaucuGGACCUG-GaGGACCa -3' miRNA: 3'- cAACCUGGacacCCUGGACaC-CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 21981 | 0.69 | 0.816124 |
Target: 5'- cUUGGACCaG-GaGGACCUGcaGGACCa -3' miRNA: 3'- cAACCUGGaCaC-CCUGGACacCCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 21783 | 0.69 | 0.816124 |
Target: 5'- -cUGGACCaGaaGGACCUG-GaGGACCa -3' miRNA: 3'- caACCUGGaCacCCUGGACaC-CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 22083 | 0.71 | 0.745429 |
Target: 5'- cUUGGACCaG-GaGGACCUG-GaGGACCa -3' miRNA: 3'- cAACCUGGaCaC-CCUGGACaC-CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 23245 | 0.71 | 0.707638 |
Target: 5'- --aGGACCaGUuGGACCUG-GaGGACCa -3' miRNA: 3'- caaCCUGGaCAcCCUGGACaC-CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 28099 | 0.72 | 0.698023 |
Target: 5'- aGUUGGACCUG-GaGGACCaGcaGGACCu -3' miRNA: 3'- -CAACCUGGACaC-CCUGGaCacCCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 21901 | 0.72 | 0.688357 |
Target: 5'- --cGGACCU-UGcGGACCUG-GaGGACCa -3' miRNA: 3'- caaCCUGGAcAC-CCUGGACaC-CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 23338 | 0.72 | 0.686419 |
Target: 5'- aGUUGGACCUGgagaaccagcaGGACCU-UGaGGACCa -3' miRNA: 3'- -CAACCUGGACac---------CCUGGAcAC-CCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 21740 | 0.73 | 0.649344 |
Target: 5'- aGUUGGACCUG-GaGGACCaGcaGGACCa -3' miRNA: 3'- -CAACCUGGACaC-CCUGGaCacCCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 26800 | 0.73 | 0.649344 |
Target: 5'- aGUUGGACCUG-GuGGACCaGcaGGACCa -3' miRNA: 3'- -CAACCUGGACaC-CCUGGaCacCCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 27659 | 0.73 | 0.610126 |
Target: 5'- --aGGACCUG-GaGGACCUGcuGGACCu -3' miRNA: 3'- caaCCUGGACaC-CCUGGACacCCUGG- -5' |
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31660 | 3' | -57.1 | NC_006883.1 | + | 28268 | 0.73 | 0.600342 |
Target: 5'- -cUGGACCaGUuGGACCUG-GaGGACCa -3' miRNA: 3'- caACCUGGaCAcCCUGGACaC-CCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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