Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31669 | 3' | -56.8 | NC_006883.1 | + | 112315 | 0.66 | 0.966036 |
Target: 5'- aCUGGU-GGAUCUGGuGGUgcuaCUGGuGGa -3' miRNA: 3'- -GGUCAuCCUGGACCuCCAg---GACCuCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 23443 | 0.66 | 0.948365 |
Target: 5'- uCCuGUAuuaccuGGACCUGGuGGaCCUGuAGGa -3' miRNA: 3'- -GGuCAU------CCUGGACCuCCaGGACcUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 27548 | 0.66 | 0.948365 |
Target: 5'- aCCAccuGGACCUGGAGGaccaucuccaCCUGuAGGa -3' miRNA: 3'- -GGUcauCCUGGACCUCCa---------GGACcUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 112428 | 0.67 | 0.930478 |
Target: 5'- --cGUAGGACCUaaaGGuGGUUCUGGuGc -3' miRNA: 3'- gguCAUCCUGGA---CCuCCAGGACCuCc -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 22048 | 0.67 | 0.92547 |
Target: 5'- aCCAGUuGGACCUGuAGGaccgUCUGGAc- -3' miRNA: 3'- -GGUCAuCCUGGACcUCCa---GGACCUcc -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 26560 | 0.68 | 0.914804 |
Target: 5'- uCCAGgAGGACCUGcuGGaCCUGuAGGa -3' miRNA: 3'- -GGUCaUCCUGGACcuCCaGGACcUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 28570 | 0.68 | 0.897202 |
Target: 5'- aCCuGaAGuuCCUGGuGGaCCUGGAGGa -3' miRNA: 3'- -GGuCaUCcuGGACCuCCaGGACCUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 23925 | 0.69 | 0.884421 |
Target: 5'- aCCAGUuGGACCUGuAGGaCCUGcuGGa -3' miRNA: 3'- -GGUCAuCCUGGACcUCCaGGACcuCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 26988 | 0.69 | 0.884421 |
Target: 5'- aCCAGgAGGACCUGGAGaaCCaGcAGGa -3' miRNA: 3'- -GGUCaUCCUGGACCUCcaGGaCcUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 26803 | 0.69 | 0.849016 |
Target: 5'- aCCAGUuGGACCUGGuGGaCCaGcAGGa -3' miRNA: 3'- -GGUCAuCCUGGACCuCCaGGaCcUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 27503 | 0.7 | 0.84138 |
Target: 5'- aCCuGUAGGACCUGcuGGaCCUGuAGGa -3' miRNA: 3'- -GGuCAUCCUGGACcuCCaGGACcUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 23698 | 0.71 | 0.792188 |
Target: 5'- aCCAGauGGACCUGGAGGaCCaGcAGGa -3' miRNA: 3'- -GGUCauCCUGGACCUCCaGGaCcUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 21976 | 0.71 | 0.792188 |
Target: 5'- aCCAGgAGGACCUGcAGGaCCaGGAGa -3' miRNA: 3'- -GGUCaUCCUGGACcUCCaGGaCCUCc -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 23401 | 0.72 | 0.747553 |
Target: 5'- uCCAGUuGGACCUGuGGGaCCUGuGGGa -3' miRNA: 3'- -GGUCAuCCUGGACcUCCaGGACcUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 23305 | 0.73 | 0.69106 |
Target: 5'- aCCAGUuGGACCaccgauaccUGGAGGaCCUuGAGGa -3' miRNA: 3'- -GGUCAuCCUGG---------ACCUCCaGGAcCUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 23341 | 0.73 | 0.69106 |
Target: 5'- aCCAGUuGGACCUGGAgaaccagcaGGaCCUuGAGGa -3' miRNA: 3'- -GGUCAuCCUGGACCU---------CCaGGAcCUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 27755 | 0.73 | 0.681443 |
Target: 5'- aCCAGUuGGACCUGuGGgaccaguuGGUCCUGuGGGa -3' miRNA: 3'- -GGUCAuCCUGGAC-CU--------CCAGGACcUCC- -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 26686 | 0.73 | 0.662109 |
Target: 5'- aCCAGUuccaccaccuGGACCUGGAGGaccgUCUGGAc- -3' miRNA: 3'- -GGUCAu---------CCUGGACCUCCa---GGACCUcc -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 26713 | 0.73 | 0.642686 |
Target: 5'- uCCAGgAGGACCUGcuGGaCCUGGAGu -3' miRNA: 3'- -GGUCaUCCUGGACcuCCaGGACCUCc -5' |
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31669 | 3' | -56.8 | NC_006883.1 | + | 28297 | 0.73 | 0.642686 |
Target: 5'- aCCAGUAGGACCaguUGGAccaguuccgccuGGaccaguuggaCCUGGAGGa -3' miRNA: 3'- -GGUCAUCCUGG---ACCU------------CCa---------GGACCUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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