Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31721 | 5' | -54.4 | NC_006938.1 | + | 17485 | 0.66 | 0.739908 |
Target: 5'- -cGGACGGGCGGUcacaUCGcuggCUAUGGCu -3' miRNA: 3'- aaCUUGCUCGCCGag--AGCa---GGUACUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 35504 | 0.66 | 0.781217 |
Target: 5'- cUGGGCcAGCGGCUUgcugUCGcCCuUGGCa -3' miRNA: 3'- aACUUGcUCGCCGAG----AGCaGGuACUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 1440 | 0.66 | 0.760831 |
Target: 5'- -gGGAUGAGUGcGCUCgCGUCCAa--- -3' miRNA: 3'- aaCUUGCUCGC-CGAGaGCAGGUacug -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 53023 | 0.66 | 0.750431 |
Target: 5'- --cAGC-AGCGGCauguaCUCGUCCGUGAg -3' miRNA: 3'- aacUUGcUCGCCGa----GAGCAGGUACUg -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 55886 | 0.67 | 0.685868 |
Target: 5'- -cGAGCGAGCgagGGCUC-CGgccaggugCCcgGACa -3' miRNA: 3'- aaCUUGCUCG---CCGAGaGCa-------GGuaCUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 56156 | 0.67 | 0.718542 |
Target: 5'- aUGAACGAGauccgucagucUGGCUCUCuGUCUGacgGACu -3' miRNA: 3'- aACUUGCUC-----------GCCGAGAG-CAGGUa--CUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 36330 | 0.67 | 0.718542 |
Target: 5'- -cGcAGCGAGCGGaUCUUGccgUCGUGACa -3' miRNA: 3'- aaC-UUGCUCGCCgAGAGCa--GGUACUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 39297 | 0.67 | 0.707722 |
Target: 5'- aUGAGC-AGCGGgUCgaaGUCCAugUGGCg -3' miRNA: 3'- aACUUGcUCGCCgAGag-CAGGU--ACUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 15821 | 0.67 | 0.685868 |
Target: 5'- -aGcACGAGgccagaGGUcuggaUCUCGUCCGUGGCa -3' miRNA: 3'- aaCuUGCUCg-----CCG-----AGAGCAGGUACUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 11113 | 0.68 | 0.663803 |
Target: 5'- -gGAgGCGAGUGGUUCUCGcCC--GACa -3' miRNA: 3'- aaCU-UGCUCGCCGAGAGCaGGuaCUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 56219 | 0.68 | 0.652721 |
Target: 5'- cUGAGCcaGGaCGGuCUCUCGUCCggGAUg -3' miRNA: 3'- aACUUGc-UC-GCC-GAGAGCAGGuaCUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 14534 | 0.69 | 0.564208 |
Target: 5'- -gGGACGAGUgugccgccauuGGCUCggcgGUCUAUGACg -3' miRNA: 3'- aaCUUGCUCG-----------CCGAGag--CAGGUACUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 37174 | 0.69 | 0.597222 |
Target: 5'- aUGAucuCGGGCGGcCUCUCGcCCuggaGACu -3' miRNA: 3'- aACUu--GCUCGCC-GAGAGCaGGua--CUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 35432 | 0.7 | 0.542463 |
Target: 5'- gUGAACGAggaGUGGCUCgaCG-CCAUGAUc -3' miRNA: 3'- aACUUGCU---CGCCGAGa-GCaGGUACUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 33042 | 0.71 | 0.489464 |
Target: 5'- -aGGugGAG-GaGCUC-CGUCCGUGGCg -3' miRNA: 3'- aaCUugCUCgC-CGAGaGCAGGUACUG- -5' |
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31721 | 5' | -54.4 | NC_006938.1 | + | 54994 | 1.06 | 0.001974 |
Target: 5'- cUUGAACGAGCGGCUCUCGUCCAUGACc -3' miRNA: 3'- -AACUUGCUCGCCGAGAGCAGGUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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