Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31722 | 3' | -54.1 | NC_006938.1 | + | 1188 | 0.66 | 0.821244 |
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Target: 5'- aGCGUGG-CCuCAUGGUCAUaguguccaCUCUCAc -3' miRNA: 3'- gUGCACCuGGcGUACUAGUA--------GAGGGU- -5' |
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| 31722 | 3' | -54.1 | NC_006938.1 | + | 11174 | 0.66 | 0.821244 |
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Target: 5'- -cCGUGGGuuGaCGUcGAUCGUCUCgCCu -3' miRNA: 3'- guGCACCUggC-GUA-CUAGUAGAG-GGu -5' |
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| 31722 | 3' | -54.1 | NC_006938.1 | + | 21654 | 0.66 | 0.80244 |
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Target: 5'- gACGUGGGCCGCGcgcgGGUCcagCUgUCGg -3' miRNA: 3'- gUGCACCUGGCGUa---CUAGua-GAgGGU- -5' |
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| 31722 | 3' | -54.1 | NC_006938.1 | + | 60677 | 0.67 | 0.73159 |
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Target: 5'- aACG-GGACCucGCcacUGGUCGUCUCCUu -3' miRNA: 3'- gUGCaCCUGG--CGu--ACUAGUAGAGGGu -5' |
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| 31722 | 3' | -54.1 | NC_006938.1 | + | 29208 | 0.68 | 0.710279 |
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Target: 5'- aGCGUGGugCcucgguggcucgGCGUGAcCAUCUCUCc -3' miRNA: 3'- gUGCACCugG------------CGUACUaGUAGAGGGu -5' |
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| 31722 | 3' | -54.1 | NC_006938.1 | + | 8231 | 0.69 | 0.633853 |
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Target: 5'- aCGCGUGGACCGgGcgaccgacgacUGAcUCGgcCUCCCAc -3' miRNA: 3'- -GUGCACCUGGCgU-----------ACU-AGUa-GAGGGU- -5' |
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| 31722 | 3' | -54.1 | NC_006938.1 | + | 8060 | 0.7 | 0.58991 |
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Target: 5'- gGCGUGGcCCcucCGUGGUCGUCUCUUg -3' miRNA: 3'- gUGCACCuGGc--GUACUAGUAGAGGGu -5' |
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| 31722 | 3' | -54.1 | NC_006938.1 | + | 37638 | 0.7 | 0.557292 |
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Target: 5'- gCACGUGGAcucCCGCGUGGUCAUaugaCAg -3' miRNA: 3'- -GUGCACCU---GGCGUACUAGUAgaggGU- -5' |
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| 31722 | 3' | -54.1 | NC_006938.1 | + | 54168 | 1.09 | 0.001613 |
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Target: 5'- cCACGUGGACCGCAUGAUCAUCUCCCAg -3' miRNA: 3'- -GUGCACCUGGCGUACUAGUAGAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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