Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31725 | 5' | -52.3 | NC_006938.1 | + | 16974 | 0.66 | 0.891616 |
Target: 5'- cUCGUCGGcAGCuGgggAGCGCgggGCCGuGUCg -3' miRNA: 3'- cAGUAGCC-UCG-Ca--UCGUG---UGGCuUAG- -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 41577 | 0.66 | 0.876434 |
Target: 5'- gGUUGUCGuucAGCGaUGGCAgCGCCGggUUg -3' miRNA: 3'- -CAGUAGCc--UCGC-AUCGU-GUGGCuuAG- -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 39496 | 0.67 | 0.834053 |
Target: 5'- cGUCGUCGGAagcucgguGCGUGGCuggACGCCc---- -3' miRNA: 3'- -CAGUAGCCU--------CGCAUCG---UGUGGcuuag -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 25191 | 0.67 | 0.834053 |
Target: 5'- -cCAUCaGAGCGUuccgagcgagGGCACccgACUGGAUCa -3' miRNA: 3'- caGUAGcCUCGCA----------UCGUG---UGGCUUAG- -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 60627 | 0.67 | 0.824883 |
Target: 5'- -cCAUCGGGGCGgcgAGCucCAUCGucUCu -3' miRNA: 3'- caGUAGCCUCGCa--UCGu-GUGGCuuAG- -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 33303 | 0.67 | 0.824883 |
Target: 5'- aUCGUgUGGAcacGCGccgacaAGCGCGCCGggUCg -3' miRNA: 3'- cAGUA-GCCU---CGCa-----UCGUGUGGCuuAG- -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 1097 | 0.67 | 0.815504 |
Target: 5'- uGUCGUUGGAGuUGUc-CACGCCGAAc- -3' miRNA: 3'- -CAGUAGCCUC-GCAucGUGUGGCUUag -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 43836 | 0.69 | 0.723493 |
Target: 5'- -gCGUCGGgcAGCgGUGGCugGuCCGggUCc -3' miRNA: 3'- caGUAGCC--UCG-CAUCGugU-GGCuuAG- -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 53111 | 0.69 | 0.712641 |
Target: 5'- aUCAcggCGGAGUGggcggacgcaguUGGCGCGCCGAAc- -3' miRNA: 3'- cAGUa--GCCUCGC------------AUCGUGUGGCUUag -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 26954 | 0.7 | 0.668532 |
Target: 5'- uGUCccucCGGAGUGUGGCACugccCCGGcgCg -3' miRNA: 3'- -CAGua--GCCUCGCAUCGUGu---GGCUuaG- -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 38026 | 0.71 | 0.635037 |
Target: 5'- cUCAagGGAcGCG-AGCACGCCGAGc- -3' miRNA: 3'- cAGUagCCU-CGCaUCGUGUGGCUUag -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 35920 | 0.71 | 0.612676 |
Target: 5'- cGUCAUCGGAcCGUucgccAGCGCgGCCGAGa- -3' miRNA: 3'- -CAGUAGCCUcGCA-----UCGUG-UGGCUUag -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 50321 | 0.71 | 0.612676 |
Target: 5'- uGUCAUCuccGAGCGUGGCGCGCgcacGGUCa -3' miRNA: 3'- -CAGUAGc--CUCGCAUCGUGUGgc--UUAG- -5' |
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31725 | 5' | -52.3 | NC_006938.1 | + | 53190 | 1.1 | 0.001918 |
Target: 5'- cGUCAUCGGAGCGUAGCACACCGAAUCu -3' miRNA: 3'- -CAGUAGCCUCGCAUCGUGUGGCUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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