Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31731 | 5' | -59.2 | NC_006938.1 | + | 41483 | 0.67 | 0.443514 |
Target: 5'- cGUCGGGGACUuuGCCcaggccgcCGcGCAGCc- -3' miRNA: 3'- -UAGCUCCUGAggCGGa-------GCuCGUCGaa -5' |
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31731 | 5' | -59.2 | NC_006938.1 | + | 16874 | 0.67 | 0.433958 |
Target: 5'- -aCGAGcGcgaccuGCUCCGCucgCUUGAGCAGCUg -3' miRNA: 3'- uaGCUC-C------UGAGGCG---GAGCUCGUCGAa -5' |
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31731 | 5' | -59.2 | NC_006938.1 | + | 27620 | 0.67 | 0.433958 |
Target: 5'- gAUCuGAGuGACUCCGUCUCG-GCcGCg- -3' miRNA: 3'- -UAG-CUC-CUGAGGCGGAGCuCGuCGaa -5' |
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31731 | 5' | -59.2 | NC_006938.1 | + | 37485 | 0.68 | 0.39697 |
Target: 5'- -cCGGGGACUCCuCCgcgCGAcCAGCUg -3' miRNA: 3'- uaGCUCCUGAGGcGGa--GCUcGUCGAa -5' |
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31731 | 5' | -59.2 | NC_006938.1 | + | 21063 | 0.68 | 0.379253 |
Target: 5'- uUCGAGGGaaCCGCCUCGuAGU-GCUg -3' miRNA: 3'- uAGCUCCUgaGGCGGAGC-UCGuCGAa -5' |
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31731 | 5' | -59.2 | NC_006938.1 | + | 50779 | 0.7 | 0.277501 |
Target: 5'- -aCGAGcuccGCUCCGuCCUgGAGCAGCUc -3' miRNA: 3'- uaGCUCc---UGAGGC-GGAgCUCGUCGAa -5' |
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31731 | 5' | -59.2 | NC_006938.1 | + | 31615 | 0.76 | 0.116912 |
Target: 5'- --aGAGGGgUCCGCCuucuugagcgccagcUCGAGCAGCUUg -3' miRNA: 3'- uagCUCCUgAGGCGG---------------AGCUCGUCGAA- -5' |
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31731 | 5' | -59.2 | NC_006938.1 | + | 46548 | 1.04 | 0.000921 |
Target: 5'- gAUCGAGGACUCCGCCUCGAGCAGCUUg -3' miRNA: 3'- -UAGCUCCUGAGGCGGAGCUCGUCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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