Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31745 | 5' | -56.7 | NC_006938.1 | + | 51032 | 0.66 | 0.6433 |
Target: 5'- aCCGGGGcCUCCGccgcguuugccaGCGCCAUccugaccaUCGCCAAc -3' miRNA: 3'- -GGUCUC-GAGGUa-----------CGUGGUG--------AGCGGUU- -5' |
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31745 | 5' | -56.7 | NC_006938.1 | + | 57787 | 0.66 | 0.61262 |
Target: 5'- uCCgAGAGCUCCGU-CACguuCUCGUCGAc -3' miRNA: 3'- -GG-UCUCGAGGUAcGUGgu-GAGCGGUU- -5' |
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31745 | 5' | -56.7 | NC_006938.1 | + | 56057 | 0.66 | 0.623572 |
Target: 5'- -gGGAGUggCCGgaUGCcaGCCACUCGUCGAc -3' miRNA: 3'- ggUCUCGa-GGU--ACG--UGGUGAGCGGUU- -5' |
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31745 | 5' | -56.7 | NC_006938.1 | + | 7886 | 0.66 | 0.656436 |
Target: 5'- gUCAGAGCUCUAcG-GCUGCUCGCUc- -3' miRNA: 3'- -GGUCUCGAGGUaCgUGGUGAGCGGuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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