Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31776 | 3' | -54.6 | NC_006938.1 | + | 10791 | 0.69 | 0.630648 |
Target: 5'- aGUCCUugaaccGGAAGCCAcGCGcccUGCCGUu- -3' miRNA: 3'- gCAGGA------CCUUCGGUcUGU---ACGGCAuc -5' |
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31776 | 3' | -54.6 | NC_006938.1 | + | 5614 | 1.09 | 0.001446 |
Target: 5'- cCGUCCUGGAAGCCAGACAUGCCGUAGu -3' miRNA: 3'- -GCAGGACCUUCGGUCUGUACGGCAUC- -5' |
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31776 | 3' | -54.6 | NC_006938.1 | + | 1313 | 0.72 | 0.422124 |
Target: 5'- gGUUCUGGAGGCguGGCAUcGCCuUGGc -3' miRNA: 3'- gCAGGACCUUCGguCUGUA-CGGcAUC- -5' |
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31776 | 3' | -54.6 | NC_006938.1 | + | 260 | 0.69 | 0.575976 |
Target: 5'- uCGUCUUGGAGGCaccGCGUGCCa--- -3' miRNA: 3'- -GCAGGACCUUCGgucUGUACGGcauc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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