miRNA: 3'- -GCaGGA-CCUu--CGGUCUGUACGGCauc -5'
miRNA: 3'- -GCAGG---ACCUUCGg--UCUGUACggCAUC- -5'
miRNA: 3'- gCAGGAc-CuuCGGUCUGuaCGGCauc -5'
miRNA: 3'- -GCAGGaCCU---UCGGUCUGUa--CGgCAuc -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31776 | 3' | -54.6 | NC_006938.1 | + | 44966 | 0.69 | 0.575976 |
|
Target: 5'- cCG-CCUcGGAcgcGCCGGGCAUGCCGc-- -3' miRNA: 3'- -GCaGGA-CCUu--CGGUCUGUACGGCauc -5' |
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| 31776 | 3' | -54.6 | NC_006938.1 | + | 53550 | 0.66 | 0.779628 |
|
Target: 5'- uCGUCCcugUGGGAGCgauGGACGUGagGUGGg -3' miRNA: 3'- -GCAGG---ACCUUCGg--UCUGUACggCAUC- -5' |
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| 31776 | 3' | -54.6 | NC_006938.1 | + | 55732 | 0.69 | 0.630648 |
|
Target: 5'- uGUCCUucGggGCUGGACcgGCCGa-- -3' miRNA: 3'- gCAGGAc-CuuCGGUCUGuaCGGCauc -5' |
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| 31776 | 3' | -54.6 | NC_006938.1 | + | 56237 | 0.7 | 0.554369 |
|
Target: 5'- uCGUCCgGGAugaAGCCAGACAaugGCgGUc- -3' miRNA: 3'- -GCAGGaCCU---UCGGUCUGUa--CGgCAuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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