miRNA: 3'- gCAACCGcaggCGCUC-CUugACGAGG- -5'
miRNA: 3'- gcAACCGCAG-GCG-----------CUCCU------------UGACGAGg -5'
miRNA: 3'- gcaACCGCAGG-------------CGCUcCUUGA--CGAGG- -5'
miRNA: 3'- gCAACCGCA----GGCGCUC---CUUGaCGAGG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31777 | 3' | -56.1 | NC_006938.1 | + | 43943 | 0.66 | 0.729507 |
|
Target: 5'- gGUUGGCaca-GCGGGuGGugUGCUCUu -3' miRNA: 3'- gCAACCGcaggCGCUC-CUugACGAGG- -5' |
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| 31777 | 3' | -56.1 | NC_006938.1 | + | 48352 | 0.69 | 0.527926 |
|
Target: 5'- -aUUGGCGUCgCGCagcuuggagaaGAGGAcccucucccgugGCUGCUCg -3' miRNA: 3'- gcAACCGCAG-GCG-----------CUCCU------------UGACGAGg -5' |
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| 31777 | 3' | -56.1 | NC_006938.1 | + | 50754 | 0.7 | 0.486189 |
|
Target: 5'- --cUGGCGUCCggggcacugcgcgGCGAcGAGCUccGCUCCg -3' miRNA: 3'- gcaACCGCAGG-------------CGCUcCUUGA--CGAGG- -5' |
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| 31777 | 3' | -56.1 | NC_006938.1 | + | 51446 | 0.69 | 0.548769 |
|
Target: 5'- aGUUGGCGUgggcCUGCGAGccaGGAC-GCUUCg -3' miRNA: 3'- gCAACCGCA----GGCGCUC---CUUGaCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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