Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31777 | 3' | -56.1 | NC_006938.1 | + | 35188 | 0.67 | 0.655523 |
Target: 5'- cCGUguagGGCGgCCG-GAGGGACaGCugUCCa -3' miRNA: 3'- -GCAa---CCGCaGGCgCUCCUUGaCG--AGG- -5' |
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31777 | 3' | -56.1 | NC_006938.1 | + | 28971 | 0.66 | 0.708674 |
Target: 5'- gCGUcGGCGUCCGuCGgcaggucgagccGGGcAUUcGCUCCg -3' miRNA: 3'- -GCAaCCGCAGGC-GC------------UCCuUGA-CGAGG- -5' |
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31777 | 3' | -56.1 | NC_006938.1 | + | 25542 | 0.66 | 0.749968 |
Target: 5'- gCGUgcucGCGUCCcuugagGCGcAGGAuguugccCUGCUCCg -3' miRNA: 3'- -GCAac--CGCAGG------CGC-UCCUu------GACGAGG- -5' |
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31777 | 3' | -56.1 | NC_006938.1 | + | 5395 | 1.12 | 0.000677 |
Target: 5'- aCGUUGGCGUCCGCGAGGAACUGCUCCu -3' miRNA: 3'- -GCAACCGCAGGCGCUCCUUGACGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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