Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31785 | 3' | -60 | NC_006938.1 | + | 23818 | 0.66 | 0.482455 |
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Target: 5'- -cGG-GUCAGUUGUucgcggcaccacuCAgGGCGCGCUc -3' miRNA: 3'- uaCCuCAGUCAGCG-------------GUgCCGCGCGAu -5' |
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| 31785 | 3' | -60 | NC_006938.1 | + | 24542 | 0.66 | 0.473522 |
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Target: 5'- -cGuGAGaCAGgucCGCCGCcGCGCGCUGc -3' miRNA: 3'- uaC-CUCaGUCa--GCGGUGcCGCGCGAU- -5' |
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| 31785 | 3' | -60 | NC_006938.1 | + | 24677 | 0.66 | 0.453976 |
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Target: 5'- -cGGAGUgccCAGggaggagaaGCCGCGaGCGCGCa- -3' miRNA: 3'- uaCCUCA---GUCag-------CGGUGC-CGCGCGau -5' |
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| 31785 | 3' | -60 | NC_006938.1 | + | 11603 | 0.66 | 0.453976 |
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Target: 5'- uGUGGAuGUCGGcCGCCuu-GCGCGCc- -3' miRNA: 3'- -UACCU-CAGUCaGCGGugcCGCGCGau -5' |
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| 31785 | 3' | -60 | NC_006938.1 | + | 51928 | 0.66 | 0.434875 |
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Target: 5'- cUGGGGUCAG--GCgGCGGUGgGCUc -3' miRNA: 3'- uACCUCAGUCagCGgUGCCGCgCGAu -5' |
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| 31785 | 3' | -60 | NC_006938.1 | + | 11130 | 0.67 | 0.416248 |
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Target: 5'- cAUGGAGaUCGGcuugucgCGCCaguuguACGGCGCGUa- -3' miRNA: 3'- -UACCUC-AGUCa------GCGG------UGCCGCGCGau -5' |
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| 31785 | 3' | -60 | NC_006938.1 | + | 1826 | 0.68 | 0.346865 |
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Target: 5'- cUGGGGcCAGUUGCCAcCGGCcggaGCUGu -3' miRNA: 3'- uACCUCaGUCAGCGGU-GCCGcg--CGAU- -5' |
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| 31785 | 3' | -60 | NC_006938.1 | + | 46377 | 0.69 | 0.28616 |
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Target: 5'- cGUGGGGUCcuGGaagagcucguUCGCCACcGCGCGCa- -3' miRNA: 3'- -UACCUCAG--UC----------AGCGGUGcCGCGCGau -5' |
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| 31785 | 3' | -60 | NC_006938.1 | + | 1877 | 1.04 | 0.000724 |
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Target: 5'- gAUGGAGUCAGUCGCCACGGCGCGCUAu -3' miRNA: 3'- -UACCUCAGUCAGCGGUGCCGCGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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