miRNA: 3'- -CGGac--AGGUUAcUGGUgCUCCUCC-CG- -5'
miRNA: 3'- -CGGACAGGuuACUggUGcuCCUCCCG- -5'
miRNA: 3'- -CGGAC-------------AGGUUA-----CUGgUGCUCCUCCcg -5'
miRNA: 3'- cGGACAGGUuaCuGgUGcUCC---UCCCG- -5'
miRNA: 3'- -CGGACAGGUUACUGGUGCUCCUCCCG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31798 | 5' | -55.5 | NC_006938.1 | + | 3251 | 0.68 | 0.632705 |
|
Target: 5'- uGCCgcccUUCAAUcACCAgGAGGAGGaGCu -3' miRNA: 3'- -CGGac--AGGUUAcUGGUgCUCCUCC-CG- -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 52216 | 0.68 | 0.632705 |
|
Target: 5'- gGCCUGUUUggUGGagGC--GGAGGGCu -3' miRNA: 3'- -CGGACAGGuuACUggUGcuCCUCCCG- -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 55821 | 0.67 | 0.674869 |
|
Target: 5'- cGCCUGucugggcuucgcuUCCGGUucuacGACgGCGGGGAGGa- -3' miRNA: 3'- -CGGAC-------------AGGUUA-----CUGgUGCUCCUCCcg -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 6811 | 0.67 | 0.675944 |
|
Target: 5'- aCCUGUCCAcgGuCgACaAGGccgAGGGCu -3' miRNA: 3'- cGGACAGGUuaCuGgUGcUCC---UCCCG- -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 8318 | 1.12 | 0.000693 |
|
Target: 5'- uGCCUGUCCAAUGACCACGAGGAGGGCc -3' miRNA: 3'- -CGGACAGGUUACUGGUGCUCCUCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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