miRNA: 3'- -CGG--ACAGGuuaCUGGUGCuCCUCCCG- -5'
miRNA: 3'- -CGG------ACAGGUua--CUGGu---GCUCCUCCCG- -5'
miRNA: 3'- -CGGACAGGUuaCU-GGUgCUCCU---CCCG- -5'
miRNA: 3'- -CGGACAGGUUACUGGUgcuccUCCCG- -5'
miRNA: 3'- -CGGACAGGUUACUGGUGCUCCUCCCG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31798 | 5' | -55.5 | NC_006938.1 | + | 18274 | 0.76 | 0.239685 |
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Target: 5'- aGCUggUGUCC---GGCCGCGcGGAGGGCg -3' miRNA: 3'- -CGG--ACAGGuuaCUGGUGCuCCUCCCG- -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 3410 | 0.76 | 0.239685 |
|
Target: 5'- cGCCcaacggUGUCCAccggGACUggguCGAGGAGGGCu -3' miRNA: 3'- -CGG------ACAGGUua--CUGGu---GCUCCUCCCG- -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 23127 | 0.77 | 0.205172 |
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Target: 5'- cGCCUGUCCAggGAuCCA-GAGGAagaGGGUg -3' miRNA: 3'- -CGGACAGGUuaCU-GGUgCUCCU---CCCG- -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 5264 | 0.79 | 0.157211 |
|
Target: 5'- gGCCUGUCCAGUGGCCGa----GGGGCg -3' miRNA: 3'- -CGGACAGGUUACUGGUgcuccUCCCG- -5' |
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| 31798 | 5' | -55.5 | NC_006938.1 | + | 8318 | 1.12 | 0.000693 |
|
Target: 5'- uGCCUGUCCAAUGACCACGAGGAGGGCc -3' miRNA: 3'- -CGGACAGGUUACUGGUGCUCCUCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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