Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31800 | 3' | -53.7 | NC_006938.1 | + | 10414 | 0.67 | 0.769459 |
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Target: 5'- -cGAgGGCGAGGcGUGCUACAucgacauGGAGa -3' miRNA: 3'- ucCUgCCGCUUCuCGUGAUGUu------CCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 32656 | 0.67 | 0.78952 |
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Target: 5'- aAGGACGGCaucauccggagGAAGAcGUACgaguuCGAGGAc -3' miRNA: 3'- -UCCUGCCG-----------CUUCU-CGUGau---GUUCCUc -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 18393 | 0.66 | 0.808917 |
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Target: 5'- aAGGAcaaCGGC-AAGGGCACca-AGGGAGc -3' miRNA: 3'- -UCCU---GCCGcUUCUCGUGaugUUCCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 6117 | 0.66 | 0.817406 |
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Target: 5'- gAGGcauGgGGCGAcuaugccGGAGCcacggGCUACGAGGAc -3' miRNA: 3'- -UCC---UgCCGCU-------UCUCG-----UGAUGUUCCUc -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 61736 | 0.66 | 0.818339 |
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Target: 5'- uGGACGGgGAc-GGCgACUACcuGGAGu -3' miRNA: 3'- uCCUGCCgCUucUCG-UGAUGuuCCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 7165 | 0.66 | 0.827564 |
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Target: 5'- uGGaGACGGCGAAuGGCcgaGCUcACAAGaGAGc -3' miRNA: 3'- -UC-CUGCCGCUUcUCG---UGA-UGUUC-CUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 60145 | 0.66 | 0.827564 |
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Target: 5'- cAGGugGGCGcGGGGCAg-ACGAccauugucgccGGAGc -3' miRNA: 3'- -UCCugCCGCuUCUCGUgaUGUU-----------CCUC- -5' |
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| 31800 | 3' | -53.7 | NC_006938.1 | + | 55850 | 0.66 | 0.827564 |
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Target: 5'- -cGACGGCGggGAGgaccaGCUGCc-GGAc -3' miRNA: 3'- ucCUGCCGCuuCUCg----UGAUGuuCCUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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