Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31808 | 3' | -66.7 | NC_006938.1 | + | 26932 | 0.67 | 0.204591 |
Target: 5'- cGGCGCg-GCCcgugacuGGGCCUgUCCCuCCg -3' miRNA: 3'- uCCGCGagCGGc------CCCGGAgAGGGcGG- -5' |
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31808 | 3' | -66.7 | NC_006938.1 | + | 22542 | 0.66 | 0.240525 |
Target: 5'- aGGGUGCcgacgaacguguucUUGCCGGuGCC-CUCCuUGCCg -3' miRNA: 3'- -UCCGCG--------------AGCGGCCcCGGaGAGG-GCGG- -5' |
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31808 | 3' | -66.7 | NC_006938.1 | + | 49599 | 0.66 | 0.242251 |
Target: 5'- cAGcCGCUCGCCGaGGgauGCCUCcaaggUUCGCCg -3' miRNA: 3'- -UCcGCGAGCGGC-CC---CGGAGa----GGGCGG- -5' |
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31808 | 3' | -66.7 | NC_006938.1 | + | 31717 | 0.66 | 0.244569 |
Target: 5'- aGGGCGCgucagccagggcaGCCGGuGUCUCUuacaacguggUCCGCCa -3' miRNA: 3'- -UCCGCGag-----------CGGCCcCGGAGA----------GGGCGG- -5' |
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31808 | 3' | -66.7 | NC_006938.1 | + | 59672 | 0.66 | 0.24808 |
Target: 5'- aAGGUGCUUGCCGcGcGCUUCUgagCCGCg -3' miRNA: 3'- -UCCGCGAGCGGCcC-CGGAGAg--GGCGg -5' |
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31808 | 3' | -66.7 | NC_006938.1 | + | 3375 | 0.66 | 0.260085 |
Target: 5'- ---gGCUCGCCaGGGCUUgUCCCaguGCUg -3' miRNA: 3'- uccgCGAGCGGcCCCGGAgAGGG---CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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