Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31810 | 5' | -58.4 | NC_006938.1 | + | 26132 | 0.67 | 0.537446 |
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Target: 5'- -aGCCGGuACGGGauCCacCCUGGCCUg -3' miRNA: 3'- gaUGGUC-UGCCUgcGGauGGACCGGA- -5' |
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| 31810 | 5' | -58.4 | NC_006938.1 | + | 3508 | 0.67 | 0.537446 |
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Target: 5'- -gACCAGAUcGACGCg-GCCUGGUa- -3' miRNA: 3'- gaUGGUCUGcCUGCGgaUGGACCGga -5' |
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| 31810 | 5' | -58.4 | NC_006938.1 | + | 33812 | 0.66 | 0.542644 |
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Target: 5'- aUGCCGGACGucgguggcagguaGACGCCgcccuucuccuugACCUGGUg- -3' miRNA: 3'- gAUGGUCUGC-------------CUGCGGa------------UGGACCGga -5' |
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| 31810 | 5' | -58.4 | NC_006938.1 | + | 57690 | 0.66 | 0.583695 |
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Target: 5'- --uCCAGGCGGAgaagagcaauuucucCgaGUCUGCUUGGCCUc -3' miRNA: 3'- gauGGUCUGCCU---------------G--CGGAUGGACCGGA- -5' |
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| 31810 | 5' | -58.4 | NC_006938.1 | + | 29705 | 0.66 | 0.590073 |
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Target: 5'- gUGCCAG-CGGGCGCgggUGCa-GGCCg -3' miRNA: 3'- gAUGGUCuGCCUGCGg--AUGgaCCGGa -5' |
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| 31810 | 5' | -58.4 | NC_006938.1 | + | 34596 | 0.66 | 0.590073 |
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Target: 5'- cCUGCC-GACGGACGCCgacGCCaaGUa- -3' miRNA: 3'- -GAUGGuCUGCCUGCGGa--UGGacCGga -5' |
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| 31810 | 5' | -58.4 | NC_006938.1 | + | 27329 | 0.66 | 0.590073 |
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Target: 5'- --cCCAGACGGGCGag-ACgCUGGUCc -3' miRNA: 3'- gauGGUCUGCCUGCggaUG-GACCGGa -5' |
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| 31810 | 5' | -58.4 | NC_006938.1 | + | 7549 | 0.66 | 0.590073 |
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Target: 5'- gCUACgCggGGACGGGCaagaccacgcugGCCaagcACCUGGCCg -3' miRNA: 3'- -GAUG-G--UCUGCCUG------------CGGa---UGGACCGGa -5' |
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| 31810 | 5' | -58.4 | NC_006938.1 | + | 20174 | 0.66 | 0.600729 |
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Target: 5'- aUGCCAGACuGGAUGaUCaGCUUGGCg- -3' miRNA: 3'- gAUGGUCUG-CCUGC-GGaUGGACCGga -5' |
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| 31810 | 5' | -58.4 | NC_006938.1 | + | 29281 | 0.66 | 0.600729 |
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Target: 5'- -gGCCAG-CGGAUcCuCUACCUGGUg- -3' miRNA: 3'- gaUGGUCuGCCUGcG-GAUGGACCGga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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