Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31824 | 5' | -50.4 | NC_006938.1 | + | 29402 | 0.66 | 0.965757 |
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Target: 5'- cGGcGCaUgAUGUCGucucCGUCCCGGugGc -3' miRNA: 3'- -CCaCG-AgUACAGUua--GCAGGGCUugC- -5' |
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| 31824 | 5' | -50.4 | NC_006938.1 | + | 11660 | 0.67 | 0.944977 |
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Target: 5'- cGGUGCUC-UGUCA----UCCCGcACa -3' miRNA: 3'- -CCACGAGuACAGUuagcAGGGCuUGc -5' |
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| 31824 | 5' | -50.4 | NC_006938.1 | + | 22702 | 0.67 | 0.936885 |
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Target: 5'- cGUGgUCAUGUCcuccaacagcgauugGGUgGUCCCGGcCGg -3' miRNA: 3'- cCACgAGUACAG---------------UUAgCAGGGCUuGC- -5' |
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| 31824 | 5' | -50.4 | NC_006938.1 | + | 44066 | 0.68 | 0.897293 |
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Target: 5'- aGUGUaCGUGUCAA-CGUCCUGAu-- -3' miRNA: 3'- cCACGaGUACAGUUaGCAGGGCUugc -5' |
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| 31824 | 5' | -50.4 | NC_006938.1 | + | 1462 | 0.69 | 0.88259 |
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Target: 5'- aGGUGCUUGuagacUGUCuuggGGUUGaUCCCGAGCu -3' miRNA: 3'- -CCACGAGU-----ACAG----UUAGC-AGGGCUUGc -5' |
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| 31824 | 5' | -50.4 | NC_006938.1 | + | 34095 | 0.69 | 0.87484 |
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Target: 5'- cGGUGCcuucggCAUGUCcGUCagcagCCCGAGCc -3' miRNA: 3'- -CCACGa-----GUACAGuUAGca---GGGCUUGc -5' |
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| 31824 | 5' | -50.4 | NC_006938.1 | + | 54329 | 0.69 | 0.858575 |
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Target: 5'- aGGUGgUCAcgacgaUGUCAGUuccaaccugCGUCCCGucGACGa -3' miRNA: 3'- -CCACgAGU------ACAGUUA---------GCAGGGC--UUGC- -5' |
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| 31824 | 5' | -50.4 | NC_006938.1 | + | 21049 | 1.13 | 0.002491 |
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Target: 5'- gGGUGCUCAUGUCAAUCGUCCCGAACGg -3' miRNA: 3'- -CCACGAGUACAGUUAGCAGGGCUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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