Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31827 | 5' | -53.5 | NC_006938.1 | + | 8062 | 0.67 | 0.814785 |
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Target: 5'- cGGGuCGCCGaGCAcgAGGAUggGgaCGUCGa -3' miRNA: 3'- -CCC-GUGGC-CGU--UCUUGugCaaGCAGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 6377 | 0.66 | 0.850189 |
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Target: 5'- --aCGCUGGCGAGcACACGgaaugaUCGUCa -3' miRNA: 3'- cccGUGGCCGUUCuUGUGCa-----AGCAGc -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 58462 | 0.66 | 0.858501 |
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Target: 5'- uGGGcCAgCGGCAgcaGGAACGCGaagaaGUUGg -3' miRNA: 3'- -CCC-GUgGCCGU---UCUUGUGCaag--CAGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 654 | 0.66 | 0.858501 |
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Target: 5'- cGGCACCGGCcucccACAUGUcgaucgCGUCu -3' miRNA: 3'- cCCGUGGCCGuucu-UGUGCAa-----GCAGc -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 43307 | 0.66 | 0.866581 |
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Target: 5'- aGGCACUGGCc-GggUAgGacUCGUCGa -3' miRNA: 3'- cCCGUGGCCGuuCuuGUgCa-AGCAGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 22971 | 0.66 | 0.866581 |
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Target: 5'- uGGCAcCCGGC----GCACGUUguaGUCGg -3' miRNA: 3'- cCCGU-GGCCGuucuUGUGCAAg--CAGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 24256 | 0.66 | 0.866581 |
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Target: 5'- cGGGCACUcGCAuGGucACACGgaccaCGUCGc -3' miRNA: 3'- -CCCGUGGcCGUuCU--UGUGCaa---GCAGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 59588 | 0.66 | 0.874422 |
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Target: 5'- uGGCauccGCCGcGCcAG-ACAgGUUCGUCGu -3' miRNA: 3'- cCCG----UGGC-CGuUCuUGUgCAAGCAGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 29565 | 0.66 | 0.874422 |
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Target: 5'- -cGCGCCuGGCAgcagAGAGUugGUcUCGUCGa -3' miRNA: 3'- ccCGUGG-CCGU----UCUUGugCA-AGCAGC- -5' |
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| 31827 | 5' | -53.5 | NC_006938.1 | + | 13981 | 0.73 | 0.500088 |
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Target: 5'- uGGCGCUGGCGaauguAGAACuCGaggUCGUCGu -3' miRNA: 3'- cCCGUGGCCGU-----UCUUGuGCa--AGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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