miRNA: 3'- -CUGUGGGugUCgCGGUgCGCCGCgAC- -5'
miRNA: 3'- cuGugGGUGUCGCGGUG--CGCCGC-Gac -5'
miRNA: 3'- cUGUGGGUgucGCGGUGcGCCGCGac -5'
miRNA: 3'- cUGU----GGGUGUC-GCGG-UGCGCCGCGAc -5'
miRNA: 3'- -CUgUGGG-UG-UCGCGGUGC-GCCGCGac -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31840 | 5' | -61.9 | NC_006938.1 | + | 51921 | 0.7 | 0.232304 |
|
Target: 5'- aGACGCUCugGG-GUCAgGCGGCGgUGg -3' miRNA: 3'- -CUGUGGGugUCgCGGUgCGCCGCgAC- -5' |
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| 31840 | 5' | -61.9 | NC_006938.1 | + | 51986 | 0.7 | 0.210043 |
|
Target: 5'- -uCugCUGCAGCGCCACugGCGGCGaCa- -3' miRNA: 3'- cuGugGGUGUCGCGGUG--CGCCGC-Gac -5' |
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| 31840 | 5' | -61.9 | NC_006938.1 | + | 55275 | 0.67 | 0.341514 |
|
Target: 5'- cACGCCCA---CGCCACcCGGCGCa- -3' miRNA: 3'- cUGUGGGUgucGCGGUGcGCCGCGac -5' |
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| 31840 | 5' | -61.9 | NC_006938.1 | + | 58168 | 0.69 | 0.262885 |
|
Target: 5'- aGCAguucCUCGCGGaCGCCaACGUGGCGCUc -3' miRNA: 3'- cUGU----GGGUGUC-GCGG-UGCGCCGCGAc -5' |
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| 31840 | 5' | -61.9 | NC_006938.1 | + | 58273 | 0.7 | 0.210043 |
|
Target: 5'- gGAgACCCgACgAGCGCCACGuUGGCGUc- -3' miRNA: 3'- -CUgUGGG-UG-UCGCGGUGC-GCCGCGac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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