miRNA: 3'- cuGUGGGU--GUCGCGGu-GCGCCGCgac -5'
miRNA: 3'- cuGU-GGGUGUC-GCgg----UGCGCCGCGac -5'
miRNA: 3'- cUGUGGGUGuc-GCGgUGcgCCGCGAC- -5'
miRNA: 3'- -CUGUG-GGUGUCGc---GGUGc--GCCGCGAc -5'
miRNA: 3'- -CUGUGGGUGUCGCGGUGCGCCGCGAC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31840 | 5' | -61.9 | NC_006938.1 | + | 43228 | 0.66 | 0.382489 |
|
Target: 5'- -cUACCCGgcCAGUGCCuuCGCGGCGa-- -3' miRNA: 3'- cuGUGGGU--GUCGCGGu-GCGCCGCgac -5' |
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| 31840 | 5' | -61.9 | NC_006938.1 | + | 28198 | 0.66 | 0.391068 |
|
Target: 5'- -cCGuCCCACAGaCGacgagaGCGCGGCGCg- -3' miRNA: 3'- cuGU-GGGUGUC-GCgg----UGCGCCGCGac -5' |
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| 31840 | 5' | -61.9 | NC_006938.1 | + | 22544 | 0.66 | 0.399771 |
|
Target: 5'- aACGCCCACcucCGCgACaaGGUGCUGg -3' miRNA: 3'- cUGUGGGUGuc-GCGgUGcgCCGCGAC- -5' |
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| 31840 | 5' | -61.9 | NC_006938.1 | + | 24605 | 0.66 | 0.417543 |
|
Target: 5'- gGGCACuCCGCAGCacguCUACcucCGGCGCUa -3' miRNA: 3'- -CUGUG-GGUGUCGc---GGUGc--GCCGCGAc -5' |
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| 31840 | 5' | -61.9 | NC_006938.1 | + | 27586 | 1.08 | 0.000309 |
|
Target: 5'- gGACACCCACAGCGCCACGCGGCGCUGg -3' miRNA: 3'- -CUGUGGGUGUCGCGGUGCGCCGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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