miRNA display CGI


Results 1 - 13 of 13 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
31841 3' -48.5 NC_006938.1 + 14457 0.66 0.978657
Target:  5'- aAUGAacaaCAGCCAGCGcGAcaagcUGGUCAGAUu -3'
miRNA:   3'- -UACUg---GUCGGUUGU-CU-----ACUAGUUUGc -5'
31841 3' -48.5 NC_006938.1 + 5340 0.66 0.975953
Target:  5'- --cACCAGCCGACAGAggccaUGA-CAGAg- -3'
miRNA:   3'- uacUGGUCGGUUGUCU-----ACUaGUUUgc -5'
31841 3' -48.5 NC_006938.1 + 42241 0.66 0.975953
Target:  5'- -gGACCGGUCAACGGccGAguuguuguagcCGAGCGa -3'
miRNA:   3'- uaCUGGUCGGUUGUCuaCUa----------GUUUGC- -5'
31841 3' -48.5 NC_006938.1 + 2080 0.66 0.973005
Target:  5'- -cGACUGcGCCgAGCAGGUGGUCuuuGugGa -3'
miRNA:   3'- uaCUGGU-CGG-UUGUCUACUAGu--UugC- -5'
31841 3' -48.5 NC_006938.1 + 62039 0.66 0.973005
Target:  5'- uAUGGCCacgucugcAGUCGACAuGGUG-UCAAACGu -3'
miRNA:   3'- -UACUGG--------UCGGUUGU-CUACuAGUUUGC- -5'
31841 3' -48.5 NC_006938.1 + 35130 0.67 0.966342
Target:  5'- -gGAUCAGaagacgaCGGCGGAagUGAUCAGACu -3'
miRNA:   3'- uaCUGGUCg------GUUGUCU--ACUAGUUUGc -5'
31841 3' -48.5 NC_006938.1 + 21338 0.67 0.954297
Target:  5'- -cGACgGGUCGGCGGAggGAUC-GACGu -3'
miRNA:   3'- uaCUGgUCGGUUGUCUa-CUAGuUUGC- -5'
31841 3' -48.5 NC_006938.1 + 21381 0.67 0.954297
Target:  5'- cUGGCCgAGCCGGucCGGGUGA-CAAGCc -3'
miRNA:   3'- uACUGG-UCGGUU--GUCUACUaGUUUGc -5'
31841 3' -48.5 NC_006938.1 + 62683 0.67 0.949707
Target:  5'- gGUGuCCAGCCGgaGCAGGccacUGGUCGggagaugaGACGg -3'
miRNA:   3'- -UACuGGUCGGU--UGUCU----ACUAGU--------UUGC- -5'
31841 3' -48.5 NC_006938.1 + 15342 0.68 0.934139
Target:  5'- -cGACCGGCCAGCauccAGAgcgccUCGAACa -3'
miRNA:   3'- uaCUGGUCGGUUG----UCUacu--AGUUUGc -5'
31841 3' -48.5 NC_006938.1 + 4836 0.68 0.934139
Target:  5'- --cACCGGCCGaggaguucgGCgAGGUGGUCGAGCu -3'
miRNA:   3'- uacUGGUCGGU---------UG-UCUACUAGUUUGc -5'
31841 3' -48.5 NC_006938.1 + 61967 0.76 0.571635
Target:  5'- cGUGGCCauagAGCCAACAGAUGucuuggCAGugGa -3'
miRNA:   3'- -UACUGG----UCGGUUGUCUACua----GUUugC- -5'
31841 3' -48.5 NC_006938.1 + 28126 1.09 0.005296
Target:  5'- aAUGACCAGCCAACAGAUGAUCAAACGg -3'
miRNA:   3'- -UACUGGUCGGUUGUCUACUAGUUUGC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.