Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31896 | 5' | -56.2 | NC_006938.1 | + | 61362 | 0.76 | 0.224575 |
Target: 5'- gGAGGGCCUCGacauggaggucuuCCUCGACGGGgucagcCUAGGa -3' miRNA: 3'- gUUCCUGGAGU-------------GGAGCUGCCU------GGUCC- -5' |
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31896 | 5' | -56.2 | NC_006938.1 | + | 61133 | 0.78 | 0.173592 |
Target: 5'- gCGAGGACUUCACCUgGGaGGGCUGGGc -3' miRNA: 3'- -GUUCCUGGAGUGGAgCUgCCUGGUCC- -5' |
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31896 | 5' | -56.2 | NC_006938.1 | + | 1675 | 0.79 | 0.140203 |
Target: 5'- aCAAGGACaagGCCUgGugGGACCGGGa -3' miRNA: 3'- -GUUCCUGgagUGGAgCugCCUGGUCC- -5' |
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31896 | 5' | -56.2 | NC_006938.1 | + | 53997 | 1.09 | 0.001103 |
Target: 5'- uCAAGGACCUCACCUCGACGGACCAGGu -3' miRNA: 3'- -GUUCCUGGAGUGGAGCUGCCUGGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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