Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3191 | 5' | -50.4 | NC_001502.1 | + | 2531 | 0.66 | 0.322317 |
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Target: 5'- aUGGgacAGcCACUAGUCAuuCuGGCCCCCc -3' miRNA: 3'- cAUC---UCcGUGAUCAGUuuG-UUGGGGG- -5' |
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| 3191 | 5' | -50.4 | NC_001502.1 | + | 154 | 0.66 | 0.303255 |
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Target: 5'- gGUGGucccuGGGCAggGGUCuccaaaucccGGACgAGCCCCCa -3' miRNA: 3'- -CAUC-----UCCGUgaUCAG----------UUUG-UUGGGGG- -5' |
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| 3191 | 5' | -50.4 | NC_001502.1 | + | 2351 | 0.68 | 0.220913 |
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Target: 5'- -aAG-GGC-UUAGUCAAaucugGCAACCCCa -3' miRNA: 3'- caUCuCCGuGAUCAGUU-----UGUUGGGGg -5' |
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| 3191 | 5' | -50.4 | NC_001502.1 | + | 2171 | 0.7 | 0.147998 |
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Target: 5'- -gGGAGGUcagGGUCAGG-AGCCCCCc -3' miRNA: 3'- caUCUCCGugaUCAGUUUgUUGGGGG- -5' |
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| 3191 | 5' | -50.4 | NC_001502.1 | + | 1049 | 0.72 | 0.10475 |
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Target: 5'- -cGGGGGguCUucuguaaguaGGUCGAugaGCGGCCCCCc -3' miRNA: 3'- caUCUCCguGA----------UCAGUU---UGUUGGGGG- -5' |
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| 3191 | 5' | -50.4 | NC_001502.1 | + | 2566 | 1.12 | 0.000068 |
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Target: 5'- aGUAGAGGCACUAGUCAAACAACCCCCc -3' miRNA: 3'- -CAUCUCCGUGAUCAGUUUGUUGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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