Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32003 | 3' | -53.9 | NC_006949.1 | + | 38569 | 0.67 | 0.666429 |
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Target: 5'- aACCAGCcgUGGUUauaACCAGcgacCAGUAGCg -3' miRNA: 3'- -UGGUUGugACCGA---UGGUCa---GUCGUCG- -5' |
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| 32003 | 3' | -53.9 | NC_006949.1 | + | 18943 | 0.67 | 0.670964 |
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Target: 5'- gAUUGGCAUUGGUU-CCGGUCgcggauaacggaaacGGCAGCc -3' miRNA: 3'- -UGGUUGUGACCGAuGGUCAG---------------UCGUCG- -5' |
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| 32003 | 3' | -53.9 | NC_006949.1 | + | 14481 | 0.66 | 0.677754 |
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Target: 5'- cGCCAGCGgcGGCUACgcgagcggCAGauaagcguugcUCGGCAGCg -3' miRNA: 3'- -UGGUUGUgaCCGAUG--------GUC-----------AGUCGUCG- -5' |
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| 32003 | 3' | -53.9 | NC_006949.1 | + | 24544 | 0.66 | 0.704722 |
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Target: 5'- uACC-GCGCUGGCUAUCcauuuaacuggcaauGGgUAGCAGUu -3' miRNA: 3'- -UGGuUGUGACCGAUGG---------------UCaGUCGUCG- -5' |
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| 32003 | 3' | -53.9 | NC_006949.1 | + | 33436 | 0.66 | 0.722468 |
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Target: 5'- --aGAUGCUGGCauggcCCAGccCGGCAGCg -3' miRNA: 3'- uggUUGUGACCGau---GGUCa-GUCGUCG- -5' |
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| 32003 | 3' | -53.9 | NC_006949.1 | + | 28439 | 0.66 | 0.733437 |
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Target: 5'- uACCGACGCUGGCggaaCGGcgUAG-AGCu -3' miRNA: 3'- -UGGUUGUGACCGaug-GUCa-GUCgUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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