Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32079 | 3' | -62.3 | NC_007016.1 | + | 64989 | 0.66 | 0.548722 |
Target: 5'- gUGCUaaGGC-CGCggauuCGGCAGGCACGGc -3' miRNA: 3'- -ACGAccCCGuGCG-----GCCGUCUGUGCCu -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 130137 | 0.67 | 0.529388 |
Target: 5'- aGCcGGGGgAgGCCGGCGGcgccccGCuCGGGg -3' miRNA: 3'- aCGaCCCCgUgCGGCCGUC------UGuGCCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 119620 | 0.67 | 0.529388 |
Target: 5'- cGUUGGGGaagcggAgGCC-GCGGAUGCGGAg -3' miRNA: 3'- aCGACCCCg-----UgCGGcCGUCUGUGCCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 129430 | 0.67 | 0.529388 |
Target: 5'- gGCUGGGGUGaGCUagGGUgaGGGCugGGGu -3' miRNA: 3'- aCGACCCCGUgCGG--CCG--UCUGugCCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 129386 | 0.67 | 0.529388 |
Target: 5'- gGCUGGGGUGaGCUagGGUgaGGGCugGGGu -3' miRNA: 3'- aCGACCCCGUgCGG--CCG--UCUGugCCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 129342 | 0.67 | 0.529388 |
Target: 5'- gGCUGGGGUGaGCUagGGUgaGGGCugGGGu -3' miRNA: 3'- aCGACCCCGUgCGG--CCG--UCUGugCCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 116583 | 0.67 | 0.519814 |
Target: 5'- cGCuUGGcGGCGCgGCCGGUu--CugGGAa -3' miRNA: 3'- aCG-ACC-CCGUG-CGGCCGucuGugCCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 92717 | 0.67 | 0.519814 |
Target: 5'- aGUUGGaGCGgGCCGGU--GCGCGGGc -3' miRNA: 3'- aCGACCcCGUgCGGCCGucUGUGCCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 93023 | 0.67 | 0.500878 |
Target: 5'- aGCUGGGGCGCaGCaucGCccaGCAUGGAa -3' miRNA: 3'- aCGACCCCGUG-CGgc-CGuc-UGUGCCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 26249 | 0.67 | 0.491524 |
Target: 5'- gGCgUGGuGGCuCGCCGGCuguGGGgGCGGc -3' miRNA: 3'- aCG-ACC-CCGuGCGGCCG---UCUgUGCCu -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 130346 | 0.67 | 0.491524 |
Target: 5'- gGCUcGGGGgGCGUCGG--GGCGCGGc -3' miRNA: 3'- aCGA-CCCCgUGCGGCCguCUGUGCCu -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 115963 | 0.68 | 0.454962 |
Target: 5'- cGCccGGGGU-CGCCGGCGGGuCACcGAa -3' miRNA: 3'- aCGa-CCCCGuGCGGCCGUCU-GUGcCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 26142 | 0.68 | 0.444281 |
Target: 5'- aGggGcGGGCACGCacccgcgcggggGGCAGAgGCGGAa -3' miRNA: 3'- aCgaC-CCCGUGCGg-----------CCGUCUgUGCCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 130344 | 0.69 | 0.386563 |
Target: 5'- gGCUGGGGCccgggGCGCCucGGCGGgGC-CGGc -3' miRNA: 3'- aCGACCCCG-----UGCGG--CCGUC-UGuGCCu -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 129881 | 0.7 | 0.3474 |
Target: 5'- gGCgGGGGCcCccaggcgcccgGCCGGCGGcgGCGCGGGg -3' miRNA: 3'- aCGaCCCCGuG-----------CGGCCGUC--UGUGCCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 90025 | 0.7 | 0.339915 |
Target: 5'- -cCUGGGGCGCgGUgGGCuuACACGGGc -3' miRNA: 3'- acGACCCCGUG-CGgCCGucUGUGCCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 16949 | 0.71 | 0.318168 |
Target: 5'- cGCaGGGGUcCGCCgGGCAGGCuuuCGGu -3' miRNA: 3'- aCGaCCCCGuGCGG-CCGUCUGu--GCCu -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 71650 | 0.71 | 0.304261 |
Target: 5'- gUGaCUGuGGcGCACaCCGGCAGACACaGAu -3' miRNA: 3'- -AC-GAC-CC-CGUGcGGCCGUCUGUGcCU- -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 67394 | 0.71 | 0.297484 |
Target: 5'- aGCUGGGGCGguaaaacaacccCGCUacaGGCAGACGCu-- -3' miRNA: 3'- aCGACCCCGU------------GCGG---CCGUCUGUGccu -5' |
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32079 | 3' | -62.3 | NC_007016.1 | + | 121078 | 0.72 | 0.284284 |
Target: 5'- aGCUcgGGuGGCACGCCGGUccaguuaacAGACgguGCGGGa -3' miRNA: 3'- aCGA--CC-CCGUGCGGCCG---------UCUG---UGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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