Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32086 | 5' | -59.5 | NC_007016.1 | + | 28294 | 0.66 | 0.793074 |
Target: 5'- gCUGugUGUCgCGaCGUuCGCGCgGUCc -3' miRNA: 3'- -GACugGCAGgGCaGCAuGCGCGgCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 130175 | 0.66 | 0.784221 |
Target: 5'- -cGGCCGagCCCGgCGgcgcCGCGCC-CCg -3' miRNA: 3'- gaCUGGCa-GGGCaGCau--GCGCGGcGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 20403 | 0.66 | 0.780643 |
Target: 5'- -cGGCgGUCCCcaaagcccUGCGCCGCCa -3' miRNA: 3'- gaCUGgCAGGGcagcau--GCGCGGCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 81115 | 0.66 | 0.775239 |
Target: 5'- -cGAUUgugGUCCUGUCGUGCGCcaaCGCg -3' miRNA: 3'- gaCUGG---CAGGGCAGCAUGCGcg-GCGg -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 108678 | 0.66 | 0.766138 |
Target: 5'- -gGGCgGUCCCGgugugcacCGUGCGC-CUGCa -3' miRNA: 3'- gaCUGgCAGGGCa-------GCAUGCGcGGCGg -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 99809 | 0.66 | 0.766138 |
Target: 5'- -gGGCUGUCaaacaCGUCGUcaaACGauggaGUCGCCg -3' miRNA: 3'- gaCUGGCAGg----GCAGCA---UGCg----CGGCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 126947 | 0.67 | 0.728696 |
Target: 5'- -gGGCCGcCCCucCGcugGCGCGuuGCCu -3' miRNA: 3'- gaCUGGCaGGGcaGCa--UGCGCggCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 78610 | 0.67 | 0.728696 |
Target: 5'- aUGACCGcCCCGgccaccgaggCGUcCGCaaccaCCGCCg -3' miRNA: 3'- gACUGGCaGGGCa---------GCAuGCGc----GGCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 38900 | 0.67 | 0.709472 |
Target: 5'- -gGACCGUCCCa----ACGCGCaGCCc -3' miRNA: 3'- gaCUGGCAGGGcagcaUGCGCGgCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 63001 | 0.67 | 0.709472 |
Target: 5'- aUGACCaaaacGUucaCCUGUaaaACGCGCCGCCu -3' miRNA: 3'- gACUGG-----CA---GGGCAgcaUGCGCGGCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 30885 | 0.67 | 0.699764 |
Target: 5'- -cGGCCGUgCCGggCGgugGUGCGCgGCCc -3' miRNA: 3'- gaCUGGCAgGGCa-GCa--UGCGCGgCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 68336 | 0.67 | 0.690002 |
Target: 5'- -gGACCG-CCCG-CGaUGCGCGuUCGCg -3' miRNA: 3'- gaCUGGCaGGGCaGC-AUGCGC-GGCGg -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 120123 | 0.68 | 0.660477 |
Target: 5'- ---uCCGUUCCGcCGcgcaGCGCGCgGCCg -3' miRNA: 3'- gacuGGCAGGGCaGCa---UGCGCGgCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 130021 | 0.68 | 0.660477 |
Target: 5'- -cGGCCGgCCCGggccCGcgGC-CGCCGCCc -3' miRNA: 3'- gaCUGGCaGGGCa---GCa-UGcGCGGCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 116407 | 0.68 | 0.657511 |
Target: 5'- -gGGCCGcuggcgcggacauuUCCCagaacCGgcCGCGCCGCCa -3' miRNA: 3'- gaCUGGC--------------AGGGca---GCauGCGCGGCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 130037 | 0.68 | 0.654542 |
Target: 5'- -cGGCCGggcgccugggggccCCCGcCGg--GCGCCGCCg -3' miRNA: 3'- gaCUGGCa-------------GGGCaGCaugCGCGGCGG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 59125 | 0.68 | 0.650582 |
Target: 5'- -cGAUCGUUucaCCGUCGgggGCGCGCCu-- -3' miRNA: 3'- gaCUGGCAG---GGCAGCa--UGCGCGGcgg -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 15418 | 0.68 | 0.640674 |
Target: 5'- gUGACCG-CCUGUCacgGUGCGCGguuUCGCg -3' miRNA: 3'- gACUGGCaGGGCAG---CAUGCGC---GGCGg -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 11897 | 0.68 | 0.630759 |
Target: 5'- ---uCCGUCUCGUaGUACuGCGCCGaCCc -3' miRNA: 3'- gacuGGCAGGGCAgCAUG-CGCGGC-GG- -5' |
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32086 | 5' | -59.5 | NC_007016.1 | + | 122893 | 0.69 | 0.601048 |
Target: 5'- --aGCCGUgguguuccaccCCUGgcagugggCGUACGUGCCGCCc -3' miRNA: 3'- gacUGGCA-----------GGGCa-------GCAUGCGCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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