Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32275 | 3' | -43.9 | NC_007023.1 | + | 160102 | 0.69 | 0.999968 |
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Target: 5'- gUAuUCCAUacaucgcuaCAUCACAAUaCAGCGCAc -3' miRNA: 3'- gAUuAGGUAg--------GUAGUGUUA-GUUGUGU- -5' |
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| 32275 | 3' | -43.9 | NC_007023.1 | + | 97669 | 0.7 | 0.999864 |
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Target: 5'- uCUAAUCCAUCCuAUCAgCGcccuuauguGUgGACGCAg -3' miRNA: 3'- -GAUUAGGUAGG-UAGU-GU---------UAgUUGUGU- -5' |
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| 32275 | 3' | -43.9 | NC_007023.1 | + | 48965 | 0.71 | 0.999822 |
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Target: 5'- ----gUCAUCCAUCACGGUUAAUAa- -3' miRNA: 3'- gauuaGGUAGGUAGUGUUAGUUGUgu -5' |
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| 32275 | 3' | -43.9 | NC_007023.1 | + | 151081 | 0.71 | 0.999625 |
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Target: 5'- -cAAUCCcaccaCCcgCACAGUCAACACc -3' miRNA: 3'- gaUUAGGua---GGuaGUGUUAGUUGUGu -5' |
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| 32275 | 3' | -43.9 | NC_007023.1 | + | 44625 | 0.72 | 0.998843 |
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Target: 5'- ---uUCCcguuuauacgggaGUCCAUCACAAUCuauACGCAu -3' miRNA: 3'- gauuAGG-------------UAGGUAGUGUUAGu--UGUGU- -5' |
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| 32275 | 3' | -43.9 | NC_007023.1 | + | 95116 | 0.73 | 0.998613 |
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Target: 5'- ----aCCAguuUCCAUCGCAcgUAACGCAu -3' miRNA: 3'- gauuaGGU---AGGUAGUGUuaGUUGUGU- -5' |
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| 32275 | 3' | -43.9 | NC_007023.1 | + | 147424 | 0.73 | 0.998585 |
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Target: 5'- uUGA-CCAUCCGUCAgaucuggaaaauuUAAUCAACACu -3' miRNA: 3'- gAUUaGGUAGGUAGU-------------GUUAGUUGUGu -5' |
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| 32275 | 3' | -43.9 | NC_007023.1 | + | 20497 | 0.74 | 0.994306 |
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Target: 5'- gCUAGUUCAUCCGUUuCAAUCAcCGCu -3' miRNA: 3'- -GAUUAGGUAGGUAGuGUUAGUuGUGu -5' |
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| 32275 | 3' | -43.9 | NC_007023.1 | + | 83822 | 0.75 | 0.99336 |
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Target: 5'- aUGAUUCaAUCCAUCAUAAUCGACu-- -3' miRNA: 3'- gAUUAGG-UAGGUAGUGUUAGUUGugu -5' |
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| 32275 | 3' | -43.9 | NC_007023.1 | + | 44880 | 1.12 | 0.035883 |
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Target: 5'- aCUAAUCCAUCCAUCACAAUCAACACAa -3' miRNA: 3'- -GAUUAGGUAGGUAGUGUUAGUUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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