Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32312 | 5' | -54 | NC_007024.1 | + | 21967 | 1.12 | 0.000763 |
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Target: 5'- aUCUCGAUGCCCAGAUUGGCGCUACGCu -3' miRNA: 3'- -AGAGCUACGGGUCUAACCGCGAUGCG- -5' |
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| 32312 | 5' | -54 | NC_007024.1 | + | 24665 | 0.72 | 0.406848 |
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Target: 5'- aUCUgGAUGCCCAGcucauuUGCUGCGCu -3' miRNA: 3'- -AGAgCUACGGGUCuaacc-GCGAUGCG- -5' |
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| 32312 | 5' | -54 | NC_007024.1 | + | 22219 | 0.69 | 0.626675 |
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Target: 5'- gUCUUGAUGCUCGGcaUGGCGCccaaGUc -3' miRNA: 3'- -AGAGCUACGGGUCuaACCGCGaug-CG- -5' |
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| 32312 | 5' | -54 | NC_007024.1 | + | 39779 | 0.68 | 0.648907 |
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Target: 5'- gCUCGAUGCCauuauGAcccuaaagcUGGCGCUuaaauugcaaGCGCa -3' miRNA: 3'- aGAGCUACGGgu---CUa--------ACCGCGA----------UGCG- -5' |
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| 32312 | 5' | -54 | NC_007024.1 | + | 14287 | 0.67 | 0.693094 |
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Target: 5'- cCUU--UGCCCAGcccUUGG-GCUGCGCc -3' miRNA: 3'- aGAGcuACGGGUCu--AACCgCGAUGCG- -5' |
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| 32312 | 5' | -54 | NC_007024.1 | + | 54877 | 0.66 | 0.757334 |
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Target: 5'- --gUGGUGCCCAGGgcaucCGCUGCGg -3' miRNA: 3'- agaGCUACGGGUCUaacc-GCGAUGCg -5' |
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| 32312 | 5' | -54 | NC_007024.1 | + | 23058 | 0.66 | 0.766627 |
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Target: 5'- uUCUUGAUGCCgGcAUUGGCacccaucGgaACGCg -3' miRNA: 3'- -AGAGCUACGGgUcUAACCG-------CgaUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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